验证数据展示
经过测试的应用
| Positive WB detected in | SK-N-SH cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16324-1-AP targets GFRA1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag9407 Product name: Recombinant human GFRA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 105-460 aa of BC014962 Sequence: SMYQSLQGNDLLEDSPYEPVNSRLSDIFRVVPFISVEHIPKGNNCLDAAKACNLDDICKKYRSAYITPCTTSVSNDVCNRRKCHKALRQFFDKVPAKHSYGMLFCSCRDIACTERRRQTIVPVCSYEEREKPNCLNLQDSCKTNYICRSRLADFFTNCQPESRSVSSCLKENYADCLLAYSGLIGTVMTPNYIDSSSLSVAPWCDCSNSGNDLEECLKFLNFFKDNTCLKNAIQAFGNGSDVTVWQPAFPVQTTTATTTTALRVKNKPLGPAGSENEIPTHVLPPCANLQAQKLKSNVSGNTHLCISNGNYEKEGLGASSHITTKSMAAPPSCGLSPLLVLVVTALSTLLSLTETS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | GDNF family receptor alpha 1 |
| 别名 | GDNF family receptor alpha 1, GDNF family receptor alpha-1, GDNF receptor alpha 1, GDNF receptor alpha-1, GDNFR |
| 计算分子量 | 460 aa, 51 kDa |
| 观测分子量 | 40-50 kDa |
| GenBank蛋白编号 | BC014962 |
| 基因名称 | GFRA1 |
| Gene ID (NCBI) | 2674 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P56159 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GFRA1, also named as GDNFRA, RETL1 and TRNR1, is a receptor for GDNF. It mediates the GDNF-induced autophosphorylation and activation of the RET receptor. GFRA1 has two isoforms with MW 45-50 kDa and 50-55 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GFRA1 antibody 16324-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
