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验证数据展示

  • WB analysis of Recombinant protein using 81835-2-RR (same clone as 81835-2-PBS)

    WB analysis of Recombinant protein using 81835-2-RR (same clone as 81835-2-PBS)

    Recombinant protein were subjected to SDS PAGE followed by western blot with 81835-2-RR (GFP tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81835-2-PBS in a different storage buffer formulation.

  • WB analysis of Transfected HEK-293 using 81835-2-RR (same clone as 81835-2-PBS)

    WB analysis of Transfected HEK-293 using 81835-2-RR (same clone as 81835-2-PBS)

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 81835-2-RR (GFP tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81835-2-PBS in a different storage buffer formulation.

  • IF Staining of Transfected COS-7 using 81835-2-RR (same clone as 81835-2-PBS)

    IF Staining of Transfected COS-7 using 81835-2-RR (same clone as 81835-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Transfected COS-7 cells using GFP tag antibody (81835-2-RR, Clone: 241614B3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81835-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81835-2-RR

    Affinity and Kinetic Characterization of 81835-2-RR

    Biolayer interferometry (BLl) kinetic assays of 81835-2-RR against Aequorea victoria GFP tag were performed. The affinity constant is 0.619 nM.

Aequorea victoria GFP tag Recombinant antibody, PBS Only

GFP tag Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 81835-2-PBS

产品说明书

CloneNo. 241614B3

宿主/亚型

Rabbit / IgG

种属反应性

aequorea victoria, recombinant protein

应用

WB, IF/ICC, Indirect ELISA

别名

241614B3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

81835-2-PBS targets GFP tag in WB, IF/ICC, Indirect ELISA applications and shows reactivity with aequorea victoria, recombinant protein samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 aequorea victoria, recombinant protein
免疫原 GFP tag fusion protein Ag2128 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 GFP tag
别名 241614B3
计算分子量 26 kDa
GenBank蛋白编号M62653
基因名称
Gene ID (NCBI)
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP42212
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm. When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy. This antibody is generated against the full-length eGFP protein. It exhibits reactivity towards variants of Aequorea victoria GFP, including S65T-GFP, RS-GFP, YFP, CFP, and eGFP.