验证数据展示
经过测试的应用
| Positive WB detected in | fetal human brain tissue, mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32902-1-AP targets GFOD1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag37656 Product name: Recombinant human GFOD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 272-390 aa of BC119005 Sequence: APEQELLVQDATPVSNSLLPEKAFSDIPSPYLRGTIKMMQAVRQAFQDQDDRRTWDGRPLTMAATFDDCLYALCVVDTIKRSSQTGEWQNIAIMTEEPELSPAYLISEAMRRSRMSLYC 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glucose-fructose oxidoreductase domain containing 1 |
| 别名 | C6orf114, EC:1.-.-.-, Glucose-fructose oxidoreductase domain-containing protein 1 |
| 计算分子量 | 390 aa, 43 kDa |
| 观测分子量 | 43 kDa |
| GenBank蛋白编号 | BC119005 |
| 基因名称 | GFOD1 |
| Gene ID (NCBI) | 54438 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9NXC2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glucose-fructose oxidoreductase domain 1 (GFOD1), may be linked to the development of ADHD. It plays a role in regulating oxidative stress in ADHD. GFOD1 may contribute to increased levels of oxidative stress specifically in the prefrontal cortex and cerebellar cortex regions and astrocytes affected by ADHD via up-regulation of the NF-κB p65/NOX2/oxidative stress axis (PMID: 40210145). There is a signal peptide at the N-terminal of this protein and the detected double strand in fetal human brain tissue is consistent with PMID: 38946427.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GFOD1 antibody 32902-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
