验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue |
| Positive IP detected in | HepG2 cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
产品信息
16941-1-AP targets GFM2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag10530 Product name: Recombinant human GFM2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 430-779 aa of BC015712 Sequence: GNIALTVGLKHTATGDTIVSSKSSALAAARRAEREGEKKHRQNNEAERLLLAGVEIPEPVFFCTIEPPSLSKQPDLEHALKCLQREDPSLKVRLDPDSGQTVLCGMGELHIEIIHDRIKREYGLETYLGPLQVAYRETILNSVRATDTLDRTLGDKRHLVTVEVEARPIETSSVMPVIEFEYAESINEGLLKVSQEAIENGIHSACLQGPLLGSPIQDVAITLHSLTIHPGTSTTMISACVSRCVQKALKKADKQVLEPLMNLEVTVARDYLSPVLADLAQRRGNIQEIQTRQDNKVVIGFVPLAEIMGYSTVLRTLTSGSATFALELSTYQAMNPQDQNTLLNRRSGLT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | G elongation factor, mitochondrial 2 |
| 别名 | EC:3.6.5.-, EF G2mt, EFG2, EFG2mt, EF-G2mt |
| 计算分子量 | 779 aa, 87 kDa |
| 观测分子量 | 87 kDa |
| GenBank蛋白编号 | BC015712 |
| 基因名称 | GFM2 |
| Gene ID (NCBI) | 84340 |
| RRID | AB_2110151 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q969S9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The GFM2 gene encodes a GTPase involved in mitochondrial protein synthesis. GFM2 mediates ribosome recycling but lacks translocation activity, whereas GFM1 catalyzes translocation during elongation.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for GFM2 antibody 16941-1-AP | Download protocol |
| IP protocol for GFM2 antibody 16941-1-AP | Download protocol |
| WB protocol for GFM2 antibody 16941-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Metab Tetracyclines promote survival and fitness in mitochondrial disease models.
| ||
EMBO J Non-canonical phosphoglycerate dehydrogenase activity promotes liver cancer growth via mitochondrial translation and respiratory metabolism | ||
Biochem Biophys Res Commun Muscle denervation reduces mitochondrial biogenesis and mitochondrial translation factor expression in mice. | ||
Physiol Rep Exercise-induced mitochondrial biogenesis coincides with the expression of mitochondrial translation factors in murine skeletal muscle. |
