验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, HL-60 cells, mouse thymus tissue, PC-3 cells, Raji cells, RAW264.7 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
14198-1-AP targets GFI1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 | GFI1 fusion protein Ag5401 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | growth factor independent 1 transcription repressor |
| 别名 | GFI 1, GFI1, Zinc finger protein 163, Zinc finger protein Gfi 1, ZNF163 |
| 计算分子量 | 45 kDa |
| 观测分子量 | 62-66 kDa |
| GenBank蛋白编号 | BC032751 |
| 基因名称 | GFI1 |
| Gene ID (NCBI) | 2672 |
| RRID | AB_2109976 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q99684 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GFI is a transcriptional repressor that is transiently induced during T-cell differentiation. It has critical roles in enhancing T helper-2 (Th2) cell expansion and in repressing induction of Th17 and CD103-positive inducible regulatory T (Treg) cells [PMID: 19188499]. GFI1 transcriptional regulator oncoprotein plays a crucial role in hematopoietic, inner ear, and pulmonary neuroendocrine cell development and governs cell processes as diverse as self-renewal of hematopoietic stem cells, proliferation, apoptosis, differentiation, cell fate specification, and oncogenesis (PMID: 18805794). The calculated molecular weight of GFI1 protein is about 45 kDa, but the modification of ubiquitinated GIF1 protein is about 62-66 kDa (PMID: 12721361).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GFI1 antibody 14198-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
