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验证数据展示

  • WB analysis using 24897-1-AP (same clone as 24897-1-PBS)

    WB analysis using 24897-1-AP (same clone as 24897-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24897-1-AP (GEMIN5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24897-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 24897-1-AP (same clone as 24897-1-PBS)

    WB analysis of HEK-293 using 24897-1-AP (same clone as 24897-1-PBS)

    WB result of GEMIN5 antibody (24897-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GEMIN5 transfected HEK-293 cells. This data was developed using the same antibody clone with 24897-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 24897-1-AP (same clone as 24897-1-PBS)

    IHC staining of mouse cerebellum using 24897-1-AP (same clone as 24897-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24897-1-AP (GEMIN5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24897-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GEMIN5 antibody included
  • IP experiment of HeLa using 24897-1-AP (same clone as 24897-1-PBS)

    IP experiment of HeLa using 24897-1-AP (same clone as 24897-1-PBS)

    IP result of anti-GEMIN5 (IP:24897-1-AP, 4ug; Detection:24897-1-AP 1:1000) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 24897-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 24897-1-AP (same clone as 24897-1-PBS)

    IF Staining of HeLa using 24897-1-AP (same clone as 24897-1-PBS)

    Immunofluorescent analysis of HeLa cells using 24897-1-AP (GEMIN5 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 24897-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GEMIN5 Polyclonal antibody, PBS Only

GEMIN5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 24897-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

Gem associated protein 5, Gem-associated protein 5

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy GEMIN5 Ready-To-Use IHC Kit. GEMIN5 primary antibody included.

产品信息

24897-1-PBS targets GEMIN5 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag19268

Product name: Recombinant human GEMIN5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1162-1508 aa of BC113614

Sequence: AVWKSIFSLDTPEQYQEAFQKLQNIKYPSATNNTPAKQLLLHICHDLTLAVLSQQMASWDEAVQALLRAVVRSYDSGSFTIMQEVYSAFLPDGCDHLRDKLGDHQSPATPAFKSLEAFFLYGRLYEFWWSLSRPCPNSSVWVRAGHRTLSVEPSQQLDTASTEETDPETSQPEPNRPSELDLRLTEEGERMLSTFKELFSEKHASLQNSQRTVAEVQETLAEMIRQHQKSQLCKSTANGPDKNEPEVEAEQPLCSSQSQCKEEKNEPLSLPELTKRLTEANQRMAKFPESIKAWPFPDVLECCLVLLLIRSHFPGCLAQEMQQQAQELLQKYGNTKTYRRHCQTFCM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 gem (nuclear organelle) associated protein 5
别名 Gem associated protein 5, Gem-associated protein 5
计算分子量 1508 aa, 169 kDa
观测分子量 168 kDa
GenBank蛋白编号BC113614
基因名称 GEMIN5
Gene ID (NCBI) 25929
RRIDAB_2879784
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TEQ6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GEMIN5 also named as Gem associated protein 5 is a 1508 amino-acid protein, which belongs to the WD repeat gemin-5 family. The molecular weight of GEMIN5 is 168 kDa GEMIN5 is a component of SMN complex, which plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. GEMIN5 is identified as the factor that allows the SMN complex to distinguish snRNAs from other cellular RNAs for snRNP biogenesis. GEMIN5 is found in nucleoplasm, nuclear bodies and cytoplasm.

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