GEFT-Specific Polyclonal antibody
GEFT-Specific Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse skeletal muscle tissue, HEK-293 cells, mouse heart tissue, mouse liver tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human pancreas tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14839-1-AP targets GEFT-Specific in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IHC, IP, WB, ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | RhoA/RAC/CDC42 exchange factor |
Synonyms | ARHGEF25, GEFT, GEFT-Specific, p63RhoGEF, RhoA/RAC/CDC42 exchange factor |
Calculated Molecular Weight | 64 kDa |
Observed Molecular Weight | 64 kDa |
GenBank Accession Number | NM_182947 |
Gene Symbol | GEFT |
Gene ID (NCBI) | 115557 |
RRID | AB_2089323 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q86VW2 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for GEFT-Specific antibody 14839-1-AP | Download protocol |
IHC protocol for GEFT-Specific antibody 14839-1-AP | Download protocol |
IP protocol for GEFT-Specific antibody 14839-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Genes Dev TNF-stimulated MAP kinase activation mediated by a Rho family GTPase signaling pathway. | ||
Circ Res p63RhoGEF couples Gα(q/11)-mediated signaling to Ca2+ sensitization of vascular smooth muscle contractility. | ||
Sci Signal RSK2 contributes to myogenic vasoconstriction of resistance arteries by activating smooth muscle myosin and the Na+/H+ exchanger. | ||
FASEB J p63RhoGEF--a key mediator of angiotensin II-dependent signaling and processes in vascular smooth muscle cells. | ||
Mol Cell Biol Modulation of muscle regeneration, myogenesis, and adipogenesis by the Rho family guanine nucleotide exchange factor GEFT. | ||
J Hypertens Increased level of p63RhoGEF and RhoA/Rho kinase activity in hypertensive patients. |