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验证数据展示

  • WB analysis using 24472-1-AP

    WB analysis using 24472-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24472-1-AP (GEF-H1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of HEK-293T using 24472-1-AP

    FC experiment of HEK-293T using 24472-1-AP

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human GEF-H1 (24472-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IHC staining of human rectal cancer using 24472-1-AP

    IHC staining of human rectal cancer using 24472-1-AP

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 24472-1-AP (GEF-H1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GEF-H1 antibody included
  • IF Staining of HepG2 using 24472-1-AP

    IF Staining of HepG2 using 24472-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GEF-H1 antibody (24472-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of MCF-7 using 24472-1-AP

    IF Staining of MCF-7 using 24472-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using GEF-H1 antibody (24472-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 24472-1-AP

    IF Staining of HeLa using 24472-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GEF-H1 antibody (24472-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse testis using 24472-1-AP

    IHC staining of mouse testis using 24472-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24472-1-AP (GEF-H1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GEF-H1 antibody included
  • IHC staining of rat testis using 24472-1-AP

    IHC staining of rat testis using 24472-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 24472-1-AP (GEF-H1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GEF-H1 antibody included

GEF-H1 Polyclonal antibody

GEF-H1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 24472-1-AP

产品说明书

引用文献(4)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA and More (3)

别名

ARHGEF2, GEF, GEF H1, GEFH1, KIAA0651

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, HEK-293T cells, HeLa cells, PC-3 cells
Positive IHC detected inhuman rectal cancer tissue, human appendicitis tissue, human stomach cancer tissue, rat testis tissue, human breast cancer tissue, mouse spleen tissue, mouse testis tissue, rat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, MCF-7 cells, HeLa cells
Positive FC (Intra) detected inHEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy GEF-H1 Ready-To-Use IHC Kit. GEF-H1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24472-1-AP targets GEF-H1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag13352

Product name: Recombinant human GEF-H1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 610-959 aa of BC020567

Sequence: PRGLFRSESLESPRGERLLQDAIREVEGLKDLLVGPGVELLLTPREPALPLEPDSGGNTSPGVTANGEARTFNGSIELCRADSDSSQRDRNGNQLRSPQEEALQRLVNLYGLLHGLQAAVAQQDTLMEARFPEGPERREKLCRANSRDGEAGRAGAAPVAPEKQATELALLQRQHALLQEELRRCRRLGEERATEAGSLEARLRESEQARALLEREAEEARRQLAALGQTEPLPAEAPWARRPVDPRRRSLPAGDALYLSFNPPQPSRGTDRLDLPVTTRSVHRNFEDRERQELGSPEERLQDSSDPDTGSEEEGSSRLSPPHSPRDFTRMQDIPEETESRDGEAVASES

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 rho/rac guanine nucleotide exchange factor (GEF) 2
别名 ARHGEF2, GEF, GEF H1, GEFH1, KIAA0651
计算分子量 112 kDa
观测分子量 111 kDa
GenBank蛋白编号BC020567
基因名称 GEF-H1/ARHGEF2
Gene ID (NCBI) 9181
RRIDAB_2879560
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92974
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Guanine nucleotide exchange factor H1 (GEF-H1) is also named ARHGEF2 and LFP40. GEF-H1, encoded by ARHGEF2 gene, is a member of the larger group of DH/PH containing GEFs (PMID: 15688002). GEFH1 is a RhoA activator that is associated with microtubules (MT) and its localization and activity are regulated, in part, by MT and fibronectin-binding integrins (PMID: 40154811). GEF-H1 is a RhoA specific MT-associated GEF, and can be activated by MT-depolymerizing agents such as nocodazole and colchicine. In epithelial cells, GEF-H1 is necessary for RhoA-dependent contractility and stress fiber formation in response to MT depolymerization (PMID: 29188751). In polarized epithelial cells, GEF-H1 is sequestered to apical junctions (AJ) by binding to the AJ structural proteins cingulin or paracingulin (PMID: 33126816).

实验方案

Product Specific Protocols
FC protocol for GEF-H1 antibody 24472-1-APDownload protocol
IF protocol for GEF-H1 antibody 24472-1-APDownload protocol
IHC protocol for GEF-H1 antibody 24472-1-APDownload protocol
WB protocol for GEF-H1 antibody 24472-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanWB,IP,CoIP

Cell Death Dis

Septin11 promotes hepatocellular carcinoma cell motility by activating RhoA to regulate cytoskeleton and cell adhesion

Authors - Lisheng Fu
View Article
humanWB,IHC

Cell Rep

Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome.

Authors - J J David Ho
View Article
humanWB,IP,IF

Cell Rep

MARK2 serves as a key regulator of host antiviral immunity through GEF-H1 phosphorylation.

Authors - Kezhen Wang
View Article
WB

Exp Neurol

AXL-mediate GEF-H1 phosphorylation was involved in microglia synapse phagocytosis in 5xFAD mice

Authors - Genyu Chen
View Article
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