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验证数据展示

  • WB analysis using 24472-1-AP

    WB analysis using 24472-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24472-1-AP (GEF-H1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 24472-1-AP

    IHC staining of human breast cancer using 24472-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24472-1-AP (GEF-H1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GEF-H1 antibody included
  • IHC staining of human breast cancer using 24472-1-AP

    IHC staining of human breast cancer using 24472-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24472-1-AP (GEF-H1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GEF-H1 antibody included
  • FC experiment of HEK-293T using 24472-1-AP

    FC experiment of HEK-293T using 24472-1-AP

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human GEF-H1 (24472-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using 24472-1-AP

    IF Staining of HepG2 using 24472-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GEF-H1 antibody (24472-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of MCF-7 using 24472-1-AP

    IF Staining of MCF-7 using 24472-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using GEF-H1 antibody (24472-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 24472-1-AP

    IF Staining of HeLa using 24472-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GEF-H1 antibody (24472-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

GEF-H1 Polyclonal antibody

GEF-H1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 24472-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, FC (Intra), ELISA and More (2)

别名

ARHGEF2, LFP40, KIAA0651, GEFH1, GEF H1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, HEK-293T cells, HeLa cells, PC-3 cells
Positive IHC detected inhuman breast cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, MCF-7 cells, HeLa cells
Positive FC (Intra) detected inHEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy GEF-H1 Ready-To-Use IHC Kit. GEF-H1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24472-1-AP targets GEF-H1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB, IHC, IP, CoIP
经测试反应性 human
文献引用反应性human, mouse
免疫原

CatNo: Ag13352

Product name: Recombinant human GEF-H1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 610-959 aa of BC020567

Sequence: PRGLFRSESLESPRGERLLQDAIREVEGLKDLLVGPGVELLLTPREPALPLEPDSGGNTSPGVTANGEARTFNGSIELCRADSDSSQRDRNGNQLRSPQEEALQRLVNLYGLLHGLQAAVAQQDTLMEARFPEGPERREKLCRANSRDGEAGRAGAAPVAPEKQATELALLQRQHALLQEELRRCRRLGEERATEAGSLEARLRESEQARALLEREAEEARRQLAALGQTEPLPAEAPWARRPVDPRRRSLPAGDALYLSFNPPQPSRGTDRLDLPVTTRSVHRNFEDRERQELGSPEERLQDSSDPDTGSEEEGSSRLSPPHSPRDFTRMQDIPEETESRDGEAVASES

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 rho/rac guanine nucleotide exchange factor (GEF) 2
别名 ARHGEF2, LFP40, KIAA0651, GEFH1, GEF H1
计算分子量 112 kDa
观测分子量 111 kDa
GenBank蛋白编号BC020567
基因名称 GEF-H1/ARHGEF2
Gene ID (NCBI) 9181
RRIDAB_2879560
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92974
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GEF-H1 antibody 24472-1-APDownload protocol
IHC protocol for GEF-H1 antibody 24472-1-APDownload protocol
IF protocol for GEF-H1 antibody 24472-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Cell Rep

Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome.

Authors - J J David Ho
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human,mouseWB,IP,CoIP

Cell Death Dis

Septin11 promotes hepatocellular carcinoma cell motility by activating RhoA to regulate cytoskeleton and cell adhesion

Authors - Lisheng Fu
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