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GEF-H1 Polyclonal antibody

GEF-H1 Polyclonal Antibody for FC, IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 24472-1-AP

Print datasheet

Synonyms

ARHGEF2, GEF, GEF H1, GEFH1, GEF-H1, KIAA0651, LFP40, Microtubule regulated Rho GEF, P40

验证数据展示

  • WB analysis using 24472-1-AP

    WB analysis using 24472-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24472-1-AP (GEF-H1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 24472-1-AP

    IHC staining of human breast cancer using 24472-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24472-1-AP (GEF-H1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 24472-1-AP

    IHC staining of human breast cancer using 24472-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24472-1-AP (GEF-H1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HEK-293T using 24472-1-AP

    FC experiment of HEK-293T using 24472-1-AP

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human GEF-H1 (24472-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using 24472-1-AP

    IF Staining of HepG2 using 24472-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GEF-H1 antibody (24472-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of MCF-7 using 24472-1-AP

    IF Staining of MCF-7 using 24472-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using GEF-H1 antibody (24472-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 24472-1-AP

    IF Staining of HeLa using 24472-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GEF-H1 antibody (24472-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA431 cells, HEK-293T cells, HeLa cells, PC-3 cells
Positive IHC detected inhuman breast cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, MCF-7 cells, HeLa cells
Positive FC detected inHEK-293T cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24472-1-AP targets GEF-H1 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen GEF-H1 fusion protein Ag13352 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name rho/rac guanine nucleotide exchange factor (GEF) 2
Synonyms ARHGEF2, GEF, GEF H1, GEFH1, GEF-H1, KIAA0651, LFP40, Microtubule regulated Rho GEF, P40
Calculated Molecular Weight 112 kDa
Observed Molecular Weight 111 kDa
GenBank Accession NumberBC020567
Gene Symbol ARHGEF2
Gene ID (NCBI) 9181
RRIDAB_2879560
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92974
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GEF-H1 antibody 24472-1-APDownload protocol
IHC protocol for GEF-H1 antibody 24472-1-APDownload protocol
IF protocol for GEF-H1 antibody 24472-1-APDownload protocol
FC protocol for GEF-H1 antibody 24472-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Cell Rep

Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome.

Authors - J J David Ho
View Article