• Featured Product
  • KD/KO验证

GDPD1 Polyclonal antibody

GDPD1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27861-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

EC:3.1.4.-, GDE4, Glycerophosphodiester phosphodiesterase domain-containing protein 1, Lysophospholipase D GDPD1, UGPQ

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inmouse testis tissue, mouse ovary tissue, rat testis tissue
Positive IHC detected inmouse testis tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27861-1-AP targets GDPD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag27371

Product name: Recombinant human GDPD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 30-195 aa of BC034432

Sequence: LHQRKKQRFLSKHISHRGGAGENLENTMAAFQHAVKIGTDMLELDCHITKDEQVVVSHDENLKRATGVNVNISDLKYCELPPYLGKLDVSFQRACQCEGKDNRIPLLKEVFEAFPNTPINIDIKVNNNVLIKKVSELVKRYNREHLTVWGNANYEIVEKCYKENSD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glycerophosphodiester phosphodiesterase domain containing 1
别名 EC:3.1.4.-, GDE4, Glycerophosphodiester phosphodiesterase domain-containing protein 1, Lysophospholipase D GDPD1, UGPQ
计算分子量 36 kDa
观测分子量 33-36 kDa
GenBank蛋白编号BC034432
基因名称 GDPD1
Gene ID (NCBI) 284161
RRIDAB_2880996
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N9F7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glycerophosphodiester phosphodiesterase domain-containing protein 1 (GDPD1) is an enzyme that hydrolyzes lysoglycerophospholipids to produce lysophosphatidic acid (LPA) and the corresponding amines. GDPD1 shows a preference for 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF), lysophosphatidylethanolamine (lyso-PE) and lysophosphatidylcholine (lyso-PC). GDPD1 may be involved in bioactive N-acylethanolamine biosynthesis from both N-acyl-lysoplasmenylethanolamin (N-acyl-lysoPlsEt) and N-acyl-lysophosphatidylethanolamin (N-acyl-lysoPE) (PMID:25596343, PMID:27637550). GDPD1 is widely expressed with a high expression level in testis. GDPD1 is a prognostic marker in Liver hepatocellular carcinoma.

实验方案

Product Specific Protocols
WB protocol for GDPD1 antibody 27861-1-APDownload protocol
IHC protocol for GDPD1 antibody 27861-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Cell Death Differ

A viral interferon regulatory factor degrades RNA-binding protein hnRNP Q1 to enhance aerobic glycolysis via recruiting E3 ubiquitin ligase KLHL3 and decaying GDPD1 mRNA.

Authors - Xiaoyu Qi
humanWB

J Lipid Res

Development of a selective fluorescence-based enzyme assay for glycerophosphodiesterase family members GDE4 and GDE7

Authors - Keisuke Kitakaze
  • KO Validated
Loading...