验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, mouse ovary tissue, rat testis tissue |
| Positive IHC detected in | mouse testis tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
27861-1-AP targets GDPD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag27371 Product name: Recombinant human GDPD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 30-195 aa of BC034432 Sequence: LHQRKKQRFLSKHISHRGGAGENLENTMAAFQHAVKIGTDMLELDCHITKDEQVVVSHDENLKRATGVNVNISDLKYCELPPYLGKLDVSFQRACQCEGKDNRIPLLKEVFEAFPNTPINIDIKVNNNVLIKKVSELVKRYNREHLTVWGNANYEIVEKCYKENSD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glycerophosphodiester phosphodiesterase domain containing 1 |
| 别名 | EC:3.1.4.-, GDE4, Glycerophosphodiester phosphodiesterase domain-containing protein 1, Lysophospholipase D GDPD1, UGPQ |
| 计算分子量 | 36 kDa |
| 观测分子量 | 33-36 kDa |
| GenBank蛋白编号 | BC034432 |
| 基因名称 | GDPD1 |
| Gene ID (NCBI) | 284161 |
| RRID | AB_2880996 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N9F7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glycerophosphodiester phosphodiesterase domain-containing protein 1 (GDPD1) is an enzyme that hydrolyzes lysoglycerophospholipids to produce lysophosphatidic acid (LPA) and the corresponding amines. GDPD1 shows a preference for 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF), lysophosphatidylethanolamine (lyso-PE) and lysophosphatidylcholine (lyso-PC). GDPD1 may be involved in bioactive N-acylethanolamine biosynthesis from both N-acyl-lysoplasmenylethanolamin (N-acyl-lysoPlsEt) and N-acyl-lysophosphatidylethanolamin (N-acyl-lysoPE) (PMID:25596343, PMID:27637550). GDPD1 is widely expressed with a high expression level in testis. GDPD1 is a prognostic marker in Liver hepatocellular carcinoma.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for GDPD1 antibody 27861-1-AP | Download protocol |
| WB protocol for GDPD1 antibody 27861-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Differ A viral interferon regulatory factor degrades RNA-binding protein hnRNP Q1 to enhance aerobic glycolysis via recruiting E3 ubiquitin ligase KLHL3 and decaying GDPD1 mRNA. | ||
J Lipid Res Development of a selective fluorescence-based enzyme assay for glycerophosphodiesterase family members GDE4 and GDE7
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