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验证数据展示

  • WB analysis using 29309-1-AP (same clone as 29309-1-PBS)

    WB analysis using 29309-1-AP (same clone as 29309-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29309-1-AP (GDF9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29309-1-PBS in a different storage buffer formulation.

  • IP experiment of A2780 using 29309-1-AP (same clone as 29309-1-PBS)

    IP experiment of A2780 using 29309-1-AP (same clone as 29309-1-PBS)

    IP result of anti-GDF9 (IP:29309-1-AP, 4ug; Detection:29309-1-AP 1:2000) with A2780 cells lysate 760 ug. This data was developed using the same antibody clone with 29309-1-PBS in a different storage buffer formulation.

  • WB analysis using 29309-1-AP (same clone as 29309-1-PBS)

    WB analysis using 29309-1-AP (same clone as 29309-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29309-1-AP (GDF9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29309-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse ovary using 29309-1-AP (same clone as 29309-1-PBS)

    IHC staining of mouse ovary using 29309-1-AP (same clone as 29309-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 29309-1-AP (GDF9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29309-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GDF9 antibody included
  • IF Staining of SKOV-3 using 29309-1-AP (same clone as 29309-1-PBS)

    IF Staining of SKOV-3 using 29309-1-AP (same clone as 29309-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using GDF9 antibody (29309-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 29309-1-PBS in a different storage buffer formulation.

GDF9 Polyclonal antibody, PBS Only

GDF9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 29309-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

GDF 9, GDF-9

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy GDF9 Ready-To-Use IHC Kit. GDF9 primary antibody included.

产品信息

29309-1-PBS targets GDF9 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag29603

Product name: Recombinant human GDF9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 400-454 aa of BC096228

Sequence: TMVQNIIYEKLDSSVPRPSCVPAKYSPLSVLTIEPDGSIAYKEYEDMIATKCTCR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 growth differentiation factor 9
别名 GDF 9, GDF-9
计算分子量 454 aa, 51 kDa
观测分子量 70 kDa
GenBank蛋白编号BC096228
基因名称 GDF9
Gene ID (NCBI) 2661
RRIDAB_3086112
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60383
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GDF9 (Growth/differentiation factor 9) belongs to the TGF-beta family. GDF9 is required for ovarian folliculogenesis. GDF9 and BMP15 are oocyte-secreted factors with a leading role in the control of ovarian function in female reproduction, modulating both the cell fate of the somatic granulosa cells and the quality and developmental competence of the egg (PMID: 29544636). GDF9 promotes cell transition from G0/G1 to S and G2/M phases, through an increase of CCND1 and CCNE1 expression, and RB1 phosphorylation (PMID: 19366876). It regulates STAR expression and cAMP-dependent progesterone release in granulosa and thecal cells. GDF9 attenuates the suppressive effects of activin A on STAR expression and progesterone production by increasing the expression of inhibin B (PMID: 21829661). It suppresses FST and FSTL3 production in granulosa-lutein cells  (PMID: 21632818, PMID: 21829661).

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