验证数据展示
经过测试的应用
| Positive WB detected in | HUVEC cells, SK-N-SH cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33521-1-AP targets GDF6/BMP-13 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag40394 Product name: Recombinant human BMP-13 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 336-455 aa of NM_001001557 Sequence: TAFASRHGKRHGKKSRLRCSKKPLHVNFKELGWDDWIIAPLEYEAYHCEGVCDFPLRSHLEPTNHAIIQTLMNSMDPGSTPPSCCVPTKLTPISILYIDAGNNVVYKQYEDMVVESCGCR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | growth differentiation factor 6 |
| 别名 | GDF6, BMP13, CDMP2, GDF 6, GDF16 |
| 计算分子量 | 51 kDa |
| 观测分子量 | 40-50 kDa |
| GenBank蛋白编号 | NM_001001557 |
| 基因名称 | GDF6 |
| Gene ID (NCBI) | 392255 |
| RRID | AB_3743000 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q6KF10 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GDF6, also known as BMP-13, is a member of the transforming growth factor-beta superfamily. GDF6 is a multifunctional growth factor that mainly functions in cells derived from the mesoderm. It can regulate the proliferation and differentiation of the retina and bones(PMID: 23307924). Mutations in GDF6 are associated with Klippel-Feil syndrome(PMID: 18425797).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GDF6/BMP-13 antibody 33521-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
