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GDEP Polyclonal antibody

GDEP Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 22744-1-AP

Print datasheet

Synonyms

GDEP, PCAN1

验证数据展示

  • WB analysis of PC-3 using 22744-1-AP

    WB analysis of PC-3 using 22744-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 22744-1-AP (GDEP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 22744-1-AP

    IHC staining of human prostate cancer using 22744-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 22744-1-AP (GDEP Antibody) at dilution of 1:50. (under 10x lens).

  • IHC staining of human prostate cancer using 22744-1-AP

    IHC staining of human prostate cancer using 22744-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 22744-1-AP (GDEP Antibody) at dilution of 1:50. (under 40x lens).

  • IF Staining of PC-3 using 22744-1-AP

    IF Staining of PC-3 using 22744-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 22744-1-AP (GDEP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human prostate cancer using 22744-1-AP

    IHC staining of human prostate cancer using 22744-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22744-1-AP (GDEP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inPC-3 cells
Positive IHC detected inhuman prostate cancer tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 22744-1-AP is GDEP Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human
Immunogen GDEP fusion protein Ag19087 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name gene differentially expressed in prostate
Synonyms GDEP, PCAN1
Calculated Molecular Weight 34 aa, 4 kDa
Observed Molecular Weight 4 kDa
GenBank Accession NumberBC125139
Gene Symbol GDEP
Gene ID (NCBI) 118425
RRIDAB_2879161
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GDEP antibody 22744-1-APDownload protocol
IHC protocol for GDEP antibody 22744-1-APDownload protocol
IF protocol for GDEP antibody 22744-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols