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验证数据展示

  • WB analysis using 13152-1-AP

    WB analysis using 13152-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13152-1-AP (GDAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 13152-1-AP

    IHC staining of mouse brain using 13152-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13152-1-AP (GDAP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 13152-1-AP

    IHC staining of mouse brain using 13152-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13152-1-AP (GDAP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GDAP1 Polyclonal antibody

GDAP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13152-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CMTRIA, GDAP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSH-SY5Y cells, human brain tissue, fetal human brain tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13152-1-AP targets GDAP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3769

Product name: Recombinant human GDAP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-257 aa of BC024939

Sequence: MRLNSTGEVPVLIHGENIICEATQIIDYLEQTFLDERTPRLMPDKESMYYPRVQHYRELLDSLPMDAYTHGCILHPELTVDSMIPAYATTRIRSQIGNTESELKKLAEENPDLQEAYIAKQKRLKSKLLDHDNVKYLKKILDELEKVLDQVETELQRRNEETPEEGQQPWLCGESFTLADVSLAVTLHRLKFLGFARRNWGNGKRPNLETYYERVLKRKTFNKVLGHVNNILISAVLPTAFRVAKKRAPKVLGTTLV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ganglioside-induced differentiation-associated protein 1
别名 CMTRIA, GDAP1
计算分子量 358 aa, 41 kDa
观测分子量 36-41 kDa
GenBank蛋白编号BC024939
基因名称 GDAP1
Gene ID (NCBI) 54332
RRIDAB_2232103
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TB36
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GDAP1 is a member of the ganglioside-induced differentiation-associated protein family, which may play a role in a signal transduction pathway during neuronal development. Mutations in this gene have been associated with various forms of Charcot-Marie-Tooth Disease and neuropathy.

实验方案

Product Specific Protocols
WB protocol for GDAP1 antibody 13152-1-APDownload protocol
IHC protocol for GDAP1 antibody 13152-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols