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验证数据展示

  • WB analysis using 13152-1-AP

    WB analysis using 13152-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13152-1-AP (GDAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 13152-1-AP

    IHC staining of mouse brain using 13152-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13152-1-AP (GDAP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 13152-1-AP

    IHC staining of mouse brain using 13152-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13152-1-AP (GDAP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GDAP1 Polyclonal antibody

GDAP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13152-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CMTRIA, GDAP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, human brain tissue, fetal human brain tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13152-1-AP targets GDAP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GDAP1 fusion protein Ag3769 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ganglioside-induced differentiation-associated protein 1
别名 CMTRIA, GDAP1
计算分子量 358 aa, 41 kDa
观测分子量 36-41 kDa
GenBank蛋白编号BC024939
基因名称 GDAP1
Gene ID (NCBI) 54332
RRIDAB_2232103
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TB36
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GDAP1 is a member of the ganglioside-induced differentiation-associated protein family, which may play a role in a signal transduction pathway during neuronal development. Mutations in this gene have been associated with various forms of Charcot-Marie-Tooth Disease and neuropathy.

实验方案

Product Specific Protocols
WB protocol for GDAP1 antibody 13152-1-APDownload protocol
IHC protocol for GDAP1 antibody 13152-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols