验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse kidney tissue, rat brain tissue, rat kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
31493-1-AP targets GDA in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag35661 Product name: Recombinant human GDA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 27-252 aa of BC053584 Sequence: EVLRDHLLGVSDSGKIVFLEEASQQEKLAKEWCFKPCEIRELSHHEFFMPGLVDTHIHASQYSFAGSSIDLPLLEWLTKYTFPAEHRFQNIDFAEEVYTRVVRRTLKNGTTTACYFATIHTDSSLLLADITDKFGQRAFVGKVCMDLNDTFPEYKETTEESIKETERFVSEMLQKNYSRVKPIVTPRFSLSCSETLMGELGNIAKTRDLHIQSHISENRDEVEAVK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | guanine deaminase |
| 别名 | Guanine aminohydrolase, Guanine aminase, Guanase, GAH, EC:3.5.4.3 |
| 观测分子量 | 48-53 kDa |
| GenBank蛋白编号 | BC053584 |
| 基因名称 | GDA |
| Gene ID (NCBI) | 9615 |
| RRID | AB_3670006 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9Y2T3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GDA ( guanine deaminase), also known as KIAA1258. The protein is enriched in the brain, intestine, kidney and liver. Cytoplasmic expression in the small intestine, hepatocytes, pancreatic islet cells and kidney tubules. This gene encodes an enzyme responsible for the hydrolytic deamination of guanine. Studies in rat ortholog suggest this gene plays a role in microtubule assembly. The molecular weight of GDA is 51 kDa, and it can recognize other isoforms.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GDA antibody 31493-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
