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验证数据展示

  • WB analysis using 17101-1-AP

    WB analysis using 17101-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17101-1-AP (GCNT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 17101-1-AP

    IHC staining of human stomach cancer using 17101-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17101-1-AP (GCNT3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GCNT3 Polyclonal antibody

GCNT3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17101-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3, C2/4GnT, C2GnT mucin type, C2GnT-M, C2GnT-mucin type

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17101-1-AP targets GCNT3 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10765

Product name: Recombinant human GCNT3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-438 aa of BC017032

Sequence: MVQWKRLCQLHYLWALGCYMLLATVALKLSFRLKCDSDHLGLESRESQSQYCRNILYNFLKLPAKRSINCSGVTRGDQEAVLQAILNNLEVKKKREPFTDTHYLSLTRDCEHFKAERKFIQFPLSKEEVEFPIAYSMVIHEKIENFERLLRAVYAPQNIYCVHVDEKSPETFKEAVKAIISCFPNVFIASKLVRVVYASWSRVQADLNCMEDLLQSSVPWKYFLNTCGTDFPIKSNAEMVQALKMLNGRNSMESEVPPKHKETRWKYHFEVVRDTLHLTNKKKDPPPYNLTMFTGNAYIVASRDFVQHVLKNPKSQQLIEWVKDTYSPDEHLWATLQRARWMPGSVPNHPKYDISDMTSIARLVKWQGHEGDIDKGAPYAPCSGIHQRAICVYGAGDLNWMLQNHHLLANKFDPKVDDNALQCLEEYLRYKAIYGTEL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glucosaminyl (N-acetyl) transferase 3, mucin type
别名 Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3, C2/4GnT, C2GnT mucin type, C2GnT-M, C2GnT-mucin type
计算分子量 438 aa, 51 kDa
观测分子量51 kDa
GenBank蛋白编号BC017032
基因名称 GCNT3
Gene ID (NCBI) 9245
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95395
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mucin-type core 2 1,6-N-acetylglucosaminyltransferase enzyme (C2GnT-M), encoded by the GCNT3 gene, has been reported to be altered in tumours and to possess tumour suppressor properties.GCNT3 is markedly expressed in both lung cancer tissues and BRCA tissues, and high GCNT3 levels correlate with poorer outcomes in patients undergoing radiotherapy (PMID: 25466507, 39697709). Elevated levels of GCNT3 promoted cell growth, migration, cell cycle development, and invasion, in addition to EMT, while suppressing apoptosis (PMID: 38789846).

实验方案

Product Specific Protocols
WB protocol for GCNT3 antibody 17101-1-APDownload protocol
IHC protocol for GCNT3 antibody 17101-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols