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验证数据展示

  • WB analysis of mouse kidney using 18118-1-AP (same clone as 18118-1-PBS)

    WB analysis of mouse kidney using 18118-1-AP (same clone as 18118-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18118-1-AP (GCNT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18118-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 18118-1-AP (same clone as 18118-1-PBS)

    IHC staining of human breast cancer using 18118-1-AP (same clone as 18118-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18118-1-AP (GCNT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18118-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GCNT2 antibody included
  • IHC staining of human liver using 18118-1-AP (same clone as 18118-1-PBS)

    IHC staining of human liver using 18118-1-AP (same clone as 18118-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18118-1-AP (GCNT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18118-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GCNT2 antibody included
  • IHC staining of human liver using 18118-1-AP (same clone as 18118-1-PBS)

    IHC staining of human liver using 18118-1-AP (same clone as 18118-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18118-1-AP (GCNT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18118-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GCNT2 antibody included
  • IP experiment of HEK-293 using 18118-1-AP (same clone as 18118-1-PBS)

    IP experiment of HEK-293 using 18118-1-AP (same clone as 18118-1-PBS)

    N/A This data was developed using the same antibody clone with 18118-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 18118-1-AP (same clone as 18118-1-PBS)

    IHC staining of mouse liver using 18118-1-AP (same clone as 18118-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 18118-1-AP (GCNT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18118-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GCNT2 antibody included

GCNT2 Polyclonal antibody, PBS Only

GCNT2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 18118-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

CCAT, EC:2.4.1.150, GCNT2C, GCNT5, I-branching enzyme

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy GCNT2 Ready-To-Use IHC Kit. GCNT2 primary antibody included.

产品信息

18118-1-PBS targets GCNT2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12635

Product name: Recombinant human GCNT2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 29-400 aa of BC074801

Sequence: GDPSFQRLNISDPLRLTQVCTSFINGKTRFLWKNKLMIHEKSSCKEYLTQSHYITAPLSKEEADFPLAYIMVIHHHFDTFARLFRAIYMPQNIYCVHVDEKATTEFKDAVEQLLSCFPNAFLASKMEPVVYGGISRLQADLNCIRDLSAFEVSWKYVINTCGQDFPLKTNKEIVQYLKGFKGKNITPGVLPPAHAIGRTKYVHQEHLGKELSYVIRTTALKPPPPHNLTIYFGSAYVALSREFANFVLHDPRAVDLLQWSKDTFSPDEHFWVTLNRIPGVPGSMPNASWTGNLRAIKWSDMEDRHGGCHGHYVHGICIYGNGDLKWLVNSPSLFANKFELNTYPLTVECLELRHRERTLNQSETAIQPSWYF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glucosaminyl (N-acetyl) transferase 2, I-branching enzyme (I blood group)
别名 CCAT, EC:2.4.1.150, GCNT2C, GCNT5, I-branching enzyme
计算分子量 400 aa, 46 kDa
观测分子量 46 kDa
GenBank蛋白编号BC074801
基因名称 GCNT2
Gene ID (NCBI) 2651
RRIDAB_2232115
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N0V5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GCNT2 (N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase) catalyzes the transfer of N-acetylglucosamine to the carbon-6 position of galactose. The loss of GCNT2 expression and corresponding loss of I-antigen in melanoma cells has profound effects on key oncogenic signaling pathways, including integrin-mediated signaling pathways critical for metastasis (PMID: 33660254, 30135430). Alterations in GCNT2 expression have now been associated with multiple malignancies, with high GCNT2 promoting tumor progression in a majority of investigated cancers. Three GCNT2 splicing variants GCNT2A, -B, and -C, which differ at exon 1 but have identical exon 2 and 3 coding regions, are expressed differentially in specific tissues (PMID: 15161861). GCNT2C determines the expression of the blood group I antigen in erythrocytes.

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