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验证数据展示

  • IHC staining of human liver cancer using 66808-1-Ig (same clone as 66808-1-PBS)

    IHC staining of human liver cancer using 66808-1-Ig (same clone as 66808-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66808-1-Ig (GCLM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66808-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66808-1-Ig (same clone as 66808-1-PBS)

    IHC staining of human liver cancer using 66808-1-Ig (same clone as 66808-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66808-1-Ig (GCLM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66808-1-PBS in a different storage buffer formulation.

  • WB analysis using 66808-1-Ig (same clone as 66808-1-PBS)

    WB analysis using 66808-1-Ig (same clone as 66808-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66808-1-Ig (GCLM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66808-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66808-1-Ig (same clone as 66808-1-PBS)

    WB analysis of HEK-293 using 66808-1-Ig (same clone as 66808-1-PBS)

    WB result of GCLM antibody (66808-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GCLM transfected HEK-293 cells. This data was developed using the same antibody clone with 66808-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

GCLM Monoclonal antibody, PBS Only

GCLM Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66808-1-PBS

产品说明书

CloneNo. 1A5A4

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

1A5A4, Gamma-ECS regulatory subunit, Gamma-glutamylcysteine synthetase regulatory subunit, GLCLR, Glutamate--cysteine ligase modifier subunit

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66808-1-PBS targets GCLM in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5497

Product name: Recombinant human GCLM protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-274 aa of BC041809

Sequence: MGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHSEELHDCIQKTLNEWSSQINPDLVREFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRRGS

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 glutamate-cysteine ligase, modifier subunit
别名 1A5A4, Gamma-ECS regulatory subunit, Gamma-glutamylcysteine synthetase regulatory subunit, GLCLR, Glutamate--cysteine ligase modifier subunit
计算分子量 31 kDa
观测分子量 31 kDa
GenBank蛋白编号BC041809
基因名称 GCLM
Gene ID (NCBI) 2730
RRIDAB_2882151
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP48507
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C