验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | rat liver tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28501-1-AP targets GCH1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag28896 Product name: Recombinant human GCH1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-250 aa of BC025415 Sequence: MEKGPVRAPAEKPRGARCSNGFPERDPPRPGPSRPAEKPPRPEAKSAQPADGWKGERPRSEEDNELNLPNLAAAYSSILSSLGENPQRQGLLKTPWRAASAMQFFTKGYQETISDVLNDAIFDEDHDEMVIVKDIDMFSMCEHHLVPFVGKVHIGYLPNKQVLGLSKLARIVEIYSRRLQVQERLTKQIAVAITEALRPAGVGVVVEATHMCMVMRGVQKMNSKTVTSTMLGVFREDPKTREEFLTLIRS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | GTP cyclohydrolase 1 |
| 别名 | EC:3.5.4.16, GTP cyclohydrolase 1, GTP cyclohydrolase I, GTPCH1, GTP-CH-I |
| 计算分子量 | 28 kDa |
| 观测分子量 | 30-35 kDa |
| GenBank蛋白编号 | BC025415 |
| 基因名称 | GCH1 |
| Gene ID (NCBI) | 2643 |
| RRID | AB_2881157 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P30793 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for GCH1 antibody 28501-1-AP | Download protocol |
| WB protocol for GCH1 antibody 28501-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Gut microbial metabolite facilitates colorectal cancer development via ferroptosis inhibition | ||
Nat Commun S100P is a ferroptosis suppressor to facilitate hepatocellular carcinoma development by rewiring lipid metabolism | ||
Nat Commun TET1 as a master regulator controlling GPX4-dependent and -independent ferroptosis surveillance in acute myeloid leukemia. | ||
Neuron Spliceosomal GTPase Eftud2 deficiency-triggered ferroptosis leads to Purkinje cell degeneration |
