验证数据展示
经过测试的应用
| Positive WB detected in | mouse pancreas tissue, rat pancreas tissue |
| Positive IHC detected in | human pancreas tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:10000-1:40000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
16317-1-AP targets GCAT in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag9367 Product name: Recombinant human GCAT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-419 aa of BC014457 Sequence: MWPGNAWRAALFWVPRGRRAQSALAQLRGILEGELEGICGAGTWKSERVITSRQGPHIRVDGVSGGILNFCANNYLGLSSHPEVIQAGLQALEEFGAGLSSVRFICGTQSIHKNLEAKIARFHQREDAILYPSCYDANAGLFEALLTPEDAVLSDELNHASIIDGIRLCKAHKYRYRHLDMADLEAKLQEAQKHRLRLVATDGAFSMDGDIAPLQEICCLASRYGALVFMDECHATGFLGPTGRGTDELLGVMDQVTIINSTLGKALGGASGGYTTGPGPLVSLLRQRARPYLFSNSLPPAVVGCASKALDLLMGSNTIVQSMAAKTQRFRSKMEAAGFTISGASHPICPVMLGDARLASRMADDMLKRGIFVIGFSYPVVPKGKAWIRVQISAVHSEEDIDRCVEAFVEVGRLHGALP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glycine C-acetyltransferase (2-amino-3-ketobutyrate coenzyme A ligase) |
| 别名 | 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial, AKB ligase, Aminoacetone synthase, EC:2.3.1.29, Glycine acetyltransferase |
| 计算分子量 | 419 aa, 45 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC014457 |
| 基因名称 | GCAT |
| Gene ID (NCBI) | 23464 |
| RRID | AB_2263046 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75600 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GCAT (Glycine acetyltransferase) is an enzyme involved in the metabolism of amino acids, specifically in the degradation of L-threonine to glycine. GCAT expression can be regulated epigenetically, and its downregulation has been linked to mitochondrial respiration defects, particularly in aging cells (PMID: 26000717).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for GCAT antibody 16317-1-AP | Download protocol |
| WB protocol for GCAT antibody 16317-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
