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验证数据展示

  • FC experiment of HepG2 using 83699-4-RR (same clone as 83699-4-PBS)

    FC experiment of HepG2 using 83699-4-RR (same clone as 83699-4-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug GBP4 Recombinant antibody (83699-4-RR, Clone:240730H5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83699-4-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 83699-4-RR (same clone as 83699-4-PBS)

    WB analysis of HeLa using 83699-4-RR (same clone as 83699-4-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 83699-4-RR (GBP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83699-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83699-4-RR

    Affinity and Kinetic Characterization of 83699-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83699-4-RR against Human GBP4 were performed. The affinity constant is 11.6 pM.

GBP4 Recombinant monoclonal antibody, PBS Only

GBP4 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83699-4-PBS

产品说明书

CloneNo. 240730H5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

240730H5, EC:3.6.5.-, GBP-4, GTP-binding protein 4, Guanine nucleotide-binding protein 4

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83699-4-PBS targets GBP4 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag12006

Product name: Recombinant human GBP4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 291-640 aa of BC070055

Sequence: AKTKTLRKGIIVTGKRLGTLVVTYVDAINSGAVPCLENAVTALAQLENPAAVQRAADHYSQQMAQQLRLPTDTLQELLDVHAACEREAIAVFMEHSFKDENHEFQKKLVDTIEKKKGDFVLQNEEASAKYCQAELKRLSEHLTESILRGIFSVPGGHNLYLEEKKQVEWDYKLVPRKGVKANEVLQNFLQSQVVVEESILQSDKALTAGEKSIAAERAMKEAAEKEQELLREKQKEQQQMMEAQERSFQEYMAQMEKKLEEERENLLREHERLLKHKLKVQEEMLKEEFQKKSEQLNKEINQLKEKIESTKNEQLRLLKILDMASNIMIVTLPGASKLLGVGTKYLGSRI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 guanylate binding protein 4
别名 240730H5, EC:3.6.5.-, GBP-4, GTP-binding protein 4, Guanine nucleotide-binding protein 4
计算分子量 640 aa, 73 kDa
观测分子量 73 kDa
GenBank蛋白编号BC070055
基因名称 GBP4
Gene ID (NCBI) 115361
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ96PP9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Guanylate-binding protein 4 (GBP4) is an IFN-inducible GTPase that belongs to the GB1/RHD3 GTPase family. GBP4 plays important roles in innate immunity against a diverse range of bacterial, viral and protozoan pathogens. GBP4 negatively regulates the antiviral response by inhibiting activation of IRF7 transcription factor. The molecular weight of GBP4 is 73 kDa.

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