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验证数据展示

  • WB analysis using 17746-1-AP

    WB analysis using 17746-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17746-1-AP (GBP4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of A431 using 17746-1-AP

    IP experiment of A431 using 17746-1-AP

    IP result of anti-GBP4 (IP:17746-1-AP, 4ug; Detection:17746-1-AP 1:500) with A431 cells lysate 1040 ug.

GBP4 Polyclonal antibody

GBP4 Polyclonal Antibody for WB, IP, ELISA
Cat No. 17746-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, ELISA

别名

Mpa2, Guanylate-binding protein 4, Guanine nucleotide-binding protein 4, GTP-binding protein 4, GBP-4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, human placenta tissue, A431 cells, mouse heart tissue
Positive IP detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17746-1-AP targets GBP4 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag12006

Product name: Recombinant human GBP4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 291-640 aa of BC070055

Sequence: AKTKTLRKGIIVTGKRLGTLVVTYVDAINSGAVPCLENAVTALAQLENPAAVQRAADHYSQQMAQQLRLPTDTLQELLDVHAACEREAIAVFMEHSFKDENHEFQKKLVDTIEKKKGDFVLQNEEASAKYCQAELKRLSEHLTESILRGIFSVPGGHNLYLEEKKQVEWDYKLVPRKGVKANEVLQNFLQSQVVVEESILQSDKALTAGEKSIAAERAMKEAAEKEQELLREKQKEQQQMMEAQERSFQEYMAQMEKKLEEERENLLREHERLLKHKLKVQEEMLKEEFQKKSEQLNKEINQLKEKIESTKNEQLRLLKILDMASNIMIVTLPGASKLLGVGTKYLGSRI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 guanylate binding protein 4
别名 Mpa2, Guanylate-binding protein 4, Guanine nucleotide-binding protein 4, GTP-binding protein 4, GBP-4
计算分子量 640 aa, 73 kDa
观测分子量 73 kDa
GenBank蛋白编号BC070055
基因名称 GBP4
Gene ID (NCBI) 115361
RRIDAB_2109346
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96PP9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Guanylate-binding protein 4 (GBP4) is an IFN-inducible GTPase that belongs to the GB1/RHD3 GTPase family. GBP4 plays important roles in innate immunity against a diverse range of bacterial, viral and protozoan pathogens. GBP4 negatively regulates the antiviral response by inhibiting activation of IRF7 transcription factor. The molecular weight of GBP4 is 73 kDa.

实验方案

Product Specific Protocols
WB protocol for GBP4 antibody 17746-1-APDownload protocol
IP protocol for GBP4 antibody 17746-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

mBio

Human GBP1 facilitates the rupture of the Legionella-containing vacuole and inflammasome activation

Authors - Antonia R Bass
View Article

PLoS One

Interleukin-27-induced HIV-resistant dendritic cells suppress reveres transcription following virus entry in an SPTBN1, autophagy, and YB-1 independent manner

Authors - Tomozumi Imamichi
View Article