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验证数据展示

  • WB analysis using 83534-1-RR

    WB analysis using 83534-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83534-1-RR (GBP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of HT-29 using 83534-1-RR

    FC experiment of HT-29 using 83534-1-RR

    1x10^6 HT-29 cells were intracellularly stained with 0.25 ug Gbp3 Recombinant Antibody (83534-1-RR, Clone:230178D8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83534-1-RR

    Affinity and Kinetic Characterization of 83534-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83534-1-RR against Human GBP3 were performed. The affinity constant is below 1 pM.

GBP3 Recombinant antibody

GBP3 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83534-1-RR

产品说明书

CloneNo. 230178D8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

GTP-binding protein 3, GTP binding protein 3, GBP-3, GBP 3, EC:3.6.5.-

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, HeLa cells, Jurkat cells, HUVEC cells, U-251 cells
Positive FC (Intra) detected inHT-29 cells
Planning an IHC experiment? We recommend our IHCeasy GBP3 Ready-To-Use IHC Kit. GBP3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83534-1-RR targets GBP3 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 GBP3 fusion protein Ag33889 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 guanylate binding protein 3
别名 GTP-binding protein 3, GTP binding protein 3, GBP-3, GBP 3, EC:3.6.5.-
观测分子量62 kDa, 68 kDa
GenBank蛋白编号BC140837
基因名称 GBP3
Gene ID (NCBI) 2635
RRIDAB_3671159
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9H0R5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Guanylate-binding protein 3 (GBP3) is involved in the proliferation of glioma cells through regulating SQSTM1-ERK1/2 pathway. GBP3 has 2 isoforms, one is 595 amino acids long at 68 kDa and the other is at nearly 62 kDa.

实验方案

Product Specific Protocols
WB protocol for GBP3 antibody 83534-1-RRDownload protocol
FC protocol for GBP3 antibody 83534-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols