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验证数据展示

  • WB analysis using 30719-1-AP

    WB analysis using 30719-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30719-1-AP (GBP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 30719-1-AP

    IHC staining of mouse cerebellum using 30719-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 30719-1-AP (GBP3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GBP3 antibody included
  • IHC staining of mouse lung using 30719-1-AP

    IHC staining of mouse lung using 30719-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30719-1-AP (GBP3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GBP3 antibody included

GBP3 Polyclonal antibody

GBP3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30719-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Guanine nucleotide-binding protein 3, GTP-binding protein 3, GTP binding protein 3, GBP-3, GBP 3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHUVEC cells
Positive IHC detected inmouse cerebellum tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GBP3 Ready-To-Use IHC Kit. GBP3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30719-1-AP targets GBP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag33889

Product name: Recombinant human GBP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 535-595 aa of BC140837

Sequence: RERAQLLEEQEKTLTSKLQEQARVLKERCQGESTQLQNEIQKLQKTLKKKTKRYMSHKLKI*

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 guanylate binding protein 3
别名 Guanine nucleotide-binding protein 3, GTP-binding protein 3, GTP binding protein 3, GBP-3, GBP 3
观测分子量68 kDa
GenBank蛋白编号BC140837
基因名称 GBP3
Gene ID (NCBI) 2635
RRIDAB_3086400
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H0R5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Guanylate-binding protein 3 (GBP3) is involved in the proliferation of glioma cells through regulating SQSTM1-ERK1/2 pathway. GBP3 has 2 isoforms, one is 595 amino acids long at 68 kDa and the other is at nearly 62 kDa.

实验方案

Product Specific Protocols
IHC protocol for GBP3 antibody 30719-1-APDownload protocol
WB protocol for GBP3 antibody 30719-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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