验证数据展示
经过测试的应用
| Positive WB detected in | A375 cells, HeLa cells, mouse spleen tissue, rat spleen tissue |
| Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 6 publications below |
| IHC | See 5 publications below |
产品信息
27299-1-AP targets GBP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag25433 Product name: Recombinant human GBP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 435-481 aa of BC022272 Sequence: TQKLQELKNKYYQVPRKGIQAKEVLKKYLESKEDVADALLQTDQSLS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | guanylate binding protein 2, interferon-inducible |
| 别名 | GBP 2, GBP2, GTP binding protein 2, HuGBP 2 |
| 计算分子量 | 591 aa, 67 kDa |
| 观测分子量 | 67 kDa |
| GenBank蛋白编号 | BC022272 |
| 基因名称 | GBP2 |
| Gene ID (NCBI) | 2634 |
| RRID | AB_2880836 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P32456 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for GBP2 antibody 27299-1-AP | Download protocol |
| WB protocol for GBP2 antibody 27299-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Immunol IRF8 Regulates Gram-Negative Bacteria-Mediated NLRP3 Inflammasome Activation and Cell Death. | ||
Anticancer Res Guanylate-binding Protein 2 Expression Is Associated With Poor Survival and Malignancy in Clear-cell Renal Cell Carcinoma.
| ||
Oncol Rep GBP2 promotes clear cell renal cell carcinoma progression through immune infiltration and regulation of PD‑L1 expression via STAT1 signaling | ||
Microvasc Res GBP2 inhibits pathological angiogenesis in the retina via the AKT/mTOR/VEGFA axis
| ||
Cancer Sci Crosstalk between GBP2 and M2 macrophage promotes the ccRCC progression
| ||
Adv Sci (Weinh) CpG-Based Nanovaccines Enhance Ovarian Cancer Immune Response by Gbp2-Mediated Remodeling of Tumor-Associated Macrophages |
