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  • Featured Product
  • KD/KO Validated

GBP2 Polyclonal antibody

GBP2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 27299-1-AP

Print datasheet

Synonyms

GBP 2, GBP2, GTP binding protein 2, HuGBP 2

验证数据展示

  • WB analysis using 27299-1-AP

    WB analysis using 27299-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27299-1-AP (GBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 27299-1-AP

    WB analysis of HeLa using 27299-1-AP

    WB result of GBP2 antibody (27299-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GBP2 transfected HeLa cells.

  • IHC staining of human spleen using 27299-1-AP

    IHC staining of human spleen using 27299-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 27299-1-AP (GBP2 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human spleen using 27299-1-AP

    IHC staining of human spleen using 27299-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 27299-1-AP (GBP2 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inA375 cells, HeLa cells, mouse spleen tissue, rat spleen tissue
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27299-1-AP targets GBP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen GBP2 fusion protein Ag25433 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name guanylate binding protein 2, interferon-inducible
Synonyms GBP 2, GBP2, GTP binding protein 2, HuGBP 2
Calculated Molecular Weight 591 aa, 67 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC022272
Gene Symbol GBP2
Gene ID (NCBI) 2634
RRIDAB_2880836
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP32456
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GBP2 antibody 27299-1-APDownload protocol
IHC protocol for GBP2 antibody 27299-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Immunol

IRF8 Regulates Gram-Negative Bacteria-Mediated NLRP3 Inflammasome Activation and Cell Death.

Authors - Rajendra Karki
View Article
humanWB,IHC

Anticancer Res

Guanylate-binding Protein 2 Expression Is Associated With Poor Survival and Malignancy in Clear-cell Renal Cell Carcinoma.

Authors - Qingyuan Liu
  • KD Validated
View Article
humanWB,IHC

Oncol Rep

GBP2 promotes clear cell renal cell carcinoma progression through immune infiltration and regulation of PD‑L1 expression via STAT1 signaling

Authors - Shujiang Ye
View Article