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验证数据展示

  • IHC staining of human liver using 23183-1-AP

    IHC staining of human liver using 23183-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23183-1-AP (GATC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 23183-1-AP

    IHC staining of human liver using 23183-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23183-1-AP (GATC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human appendicitis using 23183-1-AP

    IHC staining of human appendicitis using 23183-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 23183-1-AP (GATC antibody) at dilution of 1:400 (under 10x lens).

  • IHC staining of human appendicitis using 23183-1-AP

    IHC staining of human appendicitis using 23183-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 23183-1-AP (GATC antibody) at dilution of 1:400 (under 40x lens).

  • IP experiment of mouse liver using 23183-1-AP

    IP experiment of mouse liver using 23183-1-AP

    IP result of anti-GATC (IP:23183-1-AP, 3ug; Detection:23183-1-AP 1:500) with mouse liver tissue lysate 12500ug.

GATC Polyclonal antibody

GATC Polyclonal Antibody for IP, IHC, ELISA
Cat No. 23183-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IP, IHC, ELISA

别名

15E1.2, GATC, GatC like protein, Protein 15E1.2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue, human appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23183-1-AP targets GATC in IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IP, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 GATC fusion protein Ag19713 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glutamyl-tRNA(Gln) amidotransferase, subunit C homolog (bacterial)
别名 15E1.2, GATC, GatC like protein, Protein 15E1.2
计算分子量 136 aa, 15 kDa
观测分子量 15 kDa
GenBank蛋白编号BC107145
基因名称 GATC
Gene ID (NCBI) 283459
RRIDAB_2879226
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDO43716
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GATC encodes a subunit of the glutamyl-tRNA(Gln) amidotransferase protein complex (GatCAB), which plays a role in aminoacylation of mitochondrial glutaminyl mt-tRNA. The complex plays an role in mitochondrial translation and protein synthesis (PubMed: 30283131).

实验方案

Product Specific Protocols
IHC protocol for GATC antibody 23183-1-APDownload protocol
IP protocol for GATC antibody 23183-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols