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GATA6 Polyclonal antibody
GATA6 Polyclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat and More (1)
Applications
FC, IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, BxPC-3 cells, MCF-7 cells |
| Positive IHC detected in | human colon cancer tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | HuH-7 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 6 publications below |
| IF | See 1 publications below |
产品信息
55435-1-AP targets GATA6 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, rat samples.
| Tested Applications | FC, IHC, WB, ELISA |
| Cited Applications | WB, IF |
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | GATA binding protein 6 |
| Synonyms | GATA binding factor 6, GATA binding protein 6, GATA6, Transcription factor GATA 6 |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 60-64 kDa |
| GenBank Accession Number | NM_005257 |
| Gene Symbol | GATA6 |
| Gene ID (NCBI) | 2627 |
| RRID | AB_11182815 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92908 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GATA6 is thought to be important for regulating terminal differentiation and/or proliferation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GATA6 antibody 55435-1-AP | Download protocol |
| IHC protocol for GATA6 antibody 55435-1-AP | Download protocol |
| FC protocol for GATA6 antibody 55435-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Environ Sci Technol Perfluorinated Iodine Alkanes Promoted Neural Differentiation of mESCs by Targeting miRNA-34a-5p in Notch-Hes Signaling. | ||
iScience Commercial dishes with gelatin-free microstructured inserts for elongated stem cell self-renewal and pluripotency | ||
FASEB J Pulmonary expression of CYP2A13 and ABCB1 is regulated by FOXA2 and their genetic interaction is associated with lung cancer. | ||
Front Cell Dev Biol ADNP Controls Gene Expression Through Local Chromatin Architecture by Association With BRG1 and CHD4. | ||
Hum Cell MicroRNA-10a-5p-mediated downregulation of GATA6 inhibits tumor progression in ovarian cancer |
