验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells, HEK-293 cells, HepG2 cells, NCCIT cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68012-1-Ig targets GATA4 in WB, ELISA applications and shows reactivity with Human, Mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, Mouse |
| 免疫原 |
CatNo: Ag30392 Product name: Recombinant human GATA4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 260-442 aa of BC101580 Sequence: RLSASRRVGLSCANCQTTTTTLWRRNAEGEPVCNACGLYMKLHGVPRPLAMRKEGIQTRKRKPKNLNKSKTPAAPSGSESLPPASGASSNSSNATTSSSEEMRPIKTEPGLSSHYGHSSSVSQTFSVSAMSGHGPSIHPVLSALKLSPQGYASPVSQSPQTSSKQDSWNSLVLADSHGDIITA 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | GATA binding protein 4 |
| 别名 | GATA binding factor 4, GATA binding protein 4, GATA4, Transcription factor GATA 4 |
| 计算分子量 | 442 aa, 45 kDa |
| 观测分子量 | 44,45 kDa |
| GenBank蛋白编号 | BC101580 |
| 基因名称 | GATA4 |
| Gene ID (NCBI) | 2626 |
| RRID | AB_2918759 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P43694 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GATA4 is a transcriptional activator. GATA4 binds to the consensus sequence 5'-AGATAG-3'. GATA4 acts as a transcriptional activator of ANF in cooperation with NKX2-5. Mutation of GATA4 will cause the atrial septal defect type 2 (ASD2).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GATA4 antibody 68012-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
