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验证数据展示

  • IF Staining of HepG2 using CL488-11103

    IF Staining of HepG2 using CL488-11103

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 GATA2 antibody (CL488-11103) at dilution of 1:200.

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  • KD/KO验证

CoraLite® Plus 488-conjugated GATA2 Polyclonal antibody

GATA2 Polyclonal Antibody for IF
Cat No. CL488-11103

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF

别名

FLJ45948, GATA binding protein 2, GATA2, NFE1B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy GATA2 Ready-To-Use IHC Kit. GATA2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11103-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11103 targets GATA2 in IF applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF Application Description
经测试反应性 human, mouse, rat
免疫原 GATA2 fusion protein Ag1557 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GATA binding protein 2
别名 FLJ45948, GATA binding protein 2, GATA2, NFE1B
观测分子量50-60 kDa
GenBank蛋白编号BC018988
基因名称 GATA2
Gene ID (NCBI) 2624
ENSEMBL Gene IDENSG00000179348
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP23769
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

What is the molecular weight of GATA2? Is GATA2 post-translationally modified?

The molecular weight of GATA2 is 51 kDa. There are two known isoforms of GATA2 that use alternative first exons (PMID: 10731672). GATA2 can be phosphorylated, which regulates its activity (PMID: 7876160).

 What is the subcellular localization of GATA2?

GATA2 acts as a transcription factor in the nucleus. However, it can also be found in the cytoplasm (PMID: 25163535) and its phosphorylation promotes nuclear localization (PMID: 15837948).

 What is the tissue expression pattern of GATA2?

GATA2 was initially identified as a hematopoietic transcription factor regulating the differentiation of hematopoietic stem cells but is also present in early erythroid cells, mast cells, and megakaryocytes. Additionally, it is expressed in the central nervous system (PMID: 10357893).



实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GATA2 antibody CL488-11103Download protocol
Standard Protocols
Click here to view our Standard Protocols