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验证数据展示

  • WB analysis using 67722-1-Ig (same clone as 67722-1-PBS)

    WB analysis using 67722-1-Ig (same clone as 67722-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 67722-1-Ig (same clone as 67722-1-PBS)

    FC experiment of K-562 using 67722-1-Ig (same clone as 67722-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.5 ug Anti-Human GATA2 (67722-1-Ig, Clone:2B5A1) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 67722-1-Ig (same clone as 67722-1-PBS)

    IF Staining of SH-SY5Y using 67722-1-Ig (same clone as 67722-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using GATA2 antibody (67722-1-Ig, Clone: 2B5A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

GATA2 Monoclonal antibody, PBS Only

GATA2 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 67722-1-PBS

产品说明书

CloneNo. 2B5A1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

2B5A1, Endothelial transcription factor GATA-2, GATA binding protein 2, GATA-binding protein 2, NFE1B

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy GATA2 Ready-To-Use IHC Kit. GATA2 primary antibody included.

产品信息

67722-1-PBS targets GATA2 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1557

Product name: Recombinant human GATA2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 168-467 aa of BC018988

Sequence: SHLFGFPPTPPKEVSPDPSTTGAASPASSSAGGSAARGEDKDGVKYQVSLTESMKMESGSPLRPGLATMGTQPATHHPIPTYPSYVPAAAHDYSSGLFHPGGFLGGPASSFTPKQRSKARSCSEGRECVNCGATATPLWRRDGTGHYLCNACGLYHKMNGQNRPLIKPKRRLTTTTTLWRRNANGDPVCNACGLYYKLHNVNRPLTMKKEGIQTRNRKMSNKSKKSKKGAECFEELSKCMQEKSSPFSAAALAGHMAPVGHLPPFSHSGHILPTPTPIHPSSSLSFGHPHPSSMVTAMG

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 GATA binding protein 2
别名 2B5A1, Endothelial transcription factor GATA-2, GATA binding protein 2, GATA-binding protein 2, NFE1B
观测分子量50 kDa
GenBank蛋白编号BC018988
基因名称 GATA2
Gene ID (NCBI) 2624
ENSEMBL Gene IDENSG00000179348
RRIDAB_2882909
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP23769
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GATA2 is a member of GATA family of transcription factors that contains zinc fingers in DNA binding domain. GATA2 acts as a transcriptinal activator that regulates endothelin-1 expression in endothelial cells. Together with GATA3, GATA2 regulates adipocyte differentiation through molecular control of the preadpocyte-adipocyte transition. It also affect the activity of Rho inhibitor p190RhoGAP that controls capillary network formation in vitro and retinal angiogenesis in vivo.

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