验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, mouse testis tissue |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
10072-1-AP targets GAS7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag0114 Product name: Recombinant human GAS7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 184-382 aa of BC001152 Sequence: EEDYAKNLAKLSQNSLASQEEGSLGEAWAQVKKSLADEAEVHLKFSAKLHSEVEKPLMNFRENFKKDMKKCDHHIADLRKQLASRYASVEKARKALTERQRDLEMKTQQLEIKLSNKTEEDIKKARRKSTQAGDDLMRCVDLYNQAQSKWFEEMVTTTLELERLEVERVEMIRQHLCQYTQLRHETDMFNQSTVEPVDQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | growth arrest-specific 7 |
| 别名 | KIAA0394, Growth arrest-specific protein 7, GAS-7, GAS 7 |
| 计算分子量 | 47 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC001152 |
| 基因名称 | GAS7 |
| Gene ID (NCBI) | 8522 |
| RRID | AB_2108090 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O60861 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Growth arrest-specific (GAS) genes are expressed preferentially in cells that enter a quiescent state. Gas7, first identified in serum-starved murine fibroblasts, encodes a 48-kDa protein. 3 isoforms of Gas7 were identified in rat brains, with molecular weight of 38, 47 and 55 kDa in SDS Page.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for GAS7 antibody 10072-1-AP | Download protocol |
| WB protocol for GAS7 antibody 10072-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biomater Sci Dicalcium silicate microparticles modulate the differential expression of circRNAs and mRNAs in BMSCs and promote osteogenesis via circ_1983-miR-6931-Gas7 interaction. | ||
J Proteome Res Comparative Proteomic Analysis of Primary Schwann Cells and a Spontaneously Immortalized Schwann Cell Line RSC 96: A Comprehensive Overview with a Focus on Cell Adhesion and Migration Related Proteins. | ||
