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验证数据展示

  • WB analysis using 67181-1-Ig

    WB analysis using 67181-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67181-1-Ig (GAS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 67181-1-Ig

    IHC staining of human gliomas using 67181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67181-1-Ig (GAS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 67181-1-Ig

    IHC staining of human gliomas using 67181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67181-1-Ig (GAS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GAS1 Monoclonal antibody

GAS1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67181-1-Ig

产品说明书

CloneNo. 2H8E1

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse, Pig

应用

WB, IHC, ELISA

别名

GAS 1, GAS1, growth arrest 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, SH-SY5Y cells, pig brain tissue, Y79 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67181-1-Ig targets GAS1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Mouse, Pig
免疫原

CatNo: Ag12259

Product name: Recombinant human GAS1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 28-345 aa of BC074908

Sequence: LQLLGSAPRGSGLAHGRRLICWQALLQCQGEPECSYAYNQYAEACAPVLAQHGGGDAPGAAAAAFPASAASFSSRWRCPSHCISALIQLNHTRRGPALEDCDCAQDENCKSTKRAIEPCLPRTSGGGAGGPGAGGVMGCTEARRRCDRDSRCNLALSRYLTYCGKVFNGLRCTDECRTVIEDMLAMPKAALLNDCVCDGLERPICESVKENMARLCFGAELGNGPGSSGSDGGLDDYYDEDYDDEQRTGGAGGEQPLDDDDGVPHPPRPGSGAAASGGRGDLPYGPGRRSSGGGGRLAPRGAWTPLASILLLLLGPLF

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 growth arrest-specific 1
别名 GAS 1, GAS1, growth arrest 1
计算分子量 345 aa, 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC074908
基因名称 GAS1
Gene ID (NCBI) 2619
RRIDAB_2882477
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP54826
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

GAS1 is involved in growth suppression. It is an integral plasma membrane protein whose expression is linked to growth arrest. It can be used as a novel therapeutic candidate for gastric cancer.

实验方案

Product Specific Protocols
IHC protocol for GAS1 antibody 67181-1-IgDownload protocol
WB protocol for GAS1 antibody 67181-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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