验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17903-1-AP targets GAS1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag12275 Product name: Recombinant human GAS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 28-345 aa of BC074908 Sequence: LQLLGSAPRGSGLAHGRRLICWQALLQCQGEPECSYAYNQYAEACAPVLAQHGGGDAPGAAAAAFPASAASFSSRWRCPSHCISALIQLNHTRRGPALEDCDCAQDENCKSTKRAIEPCLPRTSGGGAGGPGAGGVMGCTEARRRCDRDSRCNLALSRYLTYCGKVFNGLRCTDECRTVIEDMLAMPKAALLNDCVCDGLERPICESVKENMARLCFGAELGNGPGSSGSDGGLDDYYDEDYDDEQRTGGAGGEQPLDDDDGVPHPPRPGSGAAASGGRGDLPYGPGRRSSGGGGRLAPRGAWTPLASILLLLLGPLF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | growth arrest-specific 1 |
| 别名 | GAS 1, GAS1, growth arrest 1 |
| 计算分子量 | 345 aa, 36 kDa |
| 观测分子量 | 36 kDa |
| GenBank蛋白编号 | BC074908 |
| 基因名称 | GAS1 |
| Gene ID (NCBI) | 2619 |
| RRID | AB_2878463 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P54826 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GAS1 is involved in growth suppression. It is an integral plasma membrane protein whose expression is linked to growth arrest. It can be used as a novel therapeutic candidate for gastric cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for GAS1 antibody 17903-1-AP | Download protocol |
| WB protocol for GAS1 antibody 17903-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Commun (Lond) Bifidobacterium adolescentis orchestrates CD143+ cancer-associated fibroblasts to suppress colorectal tumorigenesis by Wnt signaling-regulated GAS1 | ||
Sci Rep Gene-Related Response of Basal Cell Carcinoma to Biologic Treatment with Vismodegib. | ||
Exp Ther Med microRNA-mediated GAS1 downregulation promotes the proliferation of synovial fibroblasts by PI3K-Akt signaling in osteoarthritis.
| ||
FASEB Bioadv Critical cysteines in the functional interaction of adenylyl cyclase isoform 6 with Gαs. | ||
Int J Med Sci GAS1 Promotes Ferroptosis of Liver Cells in Acetaminophen-Induced Acute Liver Failure
| ||
PLoS Biol Gas1-high quiescent neural stem cells are multipotent and produce oligodendrocytes during aging and after demyelinating injury |
