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验证数据展示

  • WB analysis using 82971-1-RR (same clone as 82971-1-PBS)

    WB analysis using 82971-1-RR (same clone as 82971-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82971-1-RR (GARNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82971-1-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 82971-1-RR (same clone as 82971-1-PBS)

    FC experiment of U2OS using 82971-1-RR (same clone as 82971-1-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug GARNL1 Recombinant antibody (82971-1-RR, Clone:230165F11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82971-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 82971-1-RR (same clone as 82971-1-PBS)

    WB analysis of HEK-293 using 82971-1-RR (same clone as 82971-1-PBS)

    WB result of GARNL1 antibody (82971-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GARNL1 transfected HEK-293 cells. This data was developed using the same antibody clone with 82971-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82971-1-RR

    Affinity and Kinetic Characterization of 82971-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82971-1-RR against Human GARNL1 were performed. The affinity constant is 99.6 pM.

  • Featured Product
  • KD/KO验证

GARNL1 Recombinant monoclonal antibody, PBS Only

GARNL1 Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 82971-1-PBS

产品说明书

CloneNo. 230165F11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

230165F11, GRIPE, KIAA0884, p240, RALGAPA1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy GARNL1 Ready-To-Use IHC Kit. GARNL1 primary antibody included.

产品信息

82971-1-PBS targets GARNL1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag31518

Product name: Recombinant human GARNL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 680-820 aa of NM_014990.3

Sequence: LDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRSTRVRHFSQSEETGNEVFGALNEEQPLPRSSSTSDILEPFTVERAKVNKEDMSQK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 GTPase activating Rap/RanGAP domain-like 1
别名 230165F11, GRIPE, KIAA0884, p240, RALGAPA1
计算分子量230KD
观测分子量260 kDa
GenBank蛋白编号NM_014990.3
基因名称 GARNL1
Gene ID (NCBI) 253959
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ6GYQ0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GARNL1/RalGAPα1, a major α subunit of the Ral-GTPase activating protein in skeletal muscle, is a protein whose phosphorylation and binding to the regulatory 14-3-3 proteins is stimulated by insulin and also by muscle contraction (PMID:24768767).

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