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验证数据展示

  • WB analysis using 83577-4-RR (same clone as 83577-4-PBS)

    WB analysis using 83577-4-RR (same clone as 83577-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83577-4-RR (GAR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83577-4-PBS in a different storage buffer formulation.

  • FC experiment of K562 using 83577-4-RR (same clone as 83577-4-PBS)

    FC experiment of K562 using 83577-4-RR (same clone as 83577-4-PBS)

    1x10^6 K562 were intracellularly stained with 0.2 ug GAR1 Recombinant antibody (83577-4-RR, Clone:240537A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83577-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83577-4-RR

    Affinity and Kinetic Characterization of 83577-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83577-4-RR against Human GAR1 were performed. The affinity constant is 1.18 nM.

GAR1 Recombinant monoclonal antibody, PBS Only

GAR1 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83577-4-PBS

产品说明书

CloneNo. 240537A2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

snoRNP protein GAR1, NOLA1, 240537A2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83577-4-PBS targets GAR1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag2282

Product name: Recombinant human GAR1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-217 aa of BC003413

Sequence: MSFRGGGRGGFNRGGGGGGFNRGGSSNHFRGGGGGGGGGNFRGGGRGGFGRGGGRGGFNKGQDQGPPERVVLLGEFLHPCEDDIVCKCTTDENKVPYFNAPVYLENKEQIGKVDEIFGQLRDFYFSVKLSENMKASSFKKLQKFYIDPYKLLPLQRFLPRPPGEKGPPRGGGRGGRGGGRGGGGRGGGRGGGFRGGRGGGGGGFRGGRGGGFRGRGH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 GAR1 ribonucleoprotein homolog (yeast)
别名 snoRNP protein GAR1, NOLA1, 240537A2
计算分子量 217 aa, 22 kDa
GenBank蛋白编号BC003413
基因名称 GAR1
Gene ID (NCBI) 54433
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9NY12
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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