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GAR1 Polyclonal antibody

GAR1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 11711-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IP, IHC, ELISA and More (1)

GAR1, NOLA1, snoRNP protein GAR1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA375 cells, human skin tissue
Positive IP detected inA375 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11711-1-AP targets GAR1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IHC, IP, CoIP
经测试反应性 human
文献引用反应性human, mouse
免疫原 GAR1 fusion protein Ag2282 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GAR1 ribonucleoprotein homolog (yeast)
别名 GAR1, NOLA1, snoRNP protein GAR1
计算分子量 217 aa, 22 kDa
观测分子量 28 kDa
GenBank蛋白编号BC003413
基因名称 GAR1
Gene ID (NCBI) 54433
RRIDAB_2081845
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NY12
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GAR1, other named NOLA1, is a subunit of H/ACA and telomerase. Thought is not required for H/ACA protein assembly, GAR1 is necessary for ribosome biogenesis and telomere. H/ACA involves in class specify the sites of uridine-to-pseudouridine. It contains a core domain that flanked by glycine- and arginine-rich(GAR) domains. GAR1 also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transciptase(TERT) holoenzyme.

实验方案

Product Specific Protocols
WB protocol for GAR1 antibody 11711-1-APDownload protocol
IHC protocol for GAR1 antibody 11711-1-APDownload protocol
IP protocol for GAR1 antibody 11711-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

The human base excision repair enzyme SMUG1 directly interacts with DKC1 and contributes to RNA quality control.

Authors - Laure Jobert
humanWB

Genome Biol

Human dyskerin binds to cytoplasmic H/ACA-box-containing transcripts affecting nuclear hormone receptor dependence

Authors - Federico Zacchini
humanWB

Front Cell Dev Biol

PARP1 Regulates the Biogenesis and Activity of Telomerase Complex Through Modification of H/ACA-Proteins.

Authors - Nikita V Savelyev
humanWB

Mol Cell Biol

SUMOylation- and GAR1-Dependent Regulation of Dyskerin Nuclear and Subnuclear Localization.

Authors - D E MacNeil
humanIHC

Pathology

Expressional analysis of NOLA1, NOLA2, NOLA3 and DKC1, the core proteins in H/ACA riboproteins, in gastric and colorectal cancers.

Authors - Kim Min Sung MS
humanWB

Histochem Cell Biol

Differential requirements for H/ACA ribonucleoprotein components in cell proliferation and response to DNA damage.

Authors - Ping Lin
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