验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11711-1-AP targets GAR1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag2282 Product name: Recombinant human GAR1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-217 aa of BC003413 Sequence: MSFRGGGRGGFNRGGGGGGFNRGGSSNHFRGGGGGGGGGNFRGGGRGGFGRGGGRGGFNKGQDQGPPERVVLLGEFLHPCEDDIVCKCTTDENKVPYFNAPVYLENKEQIGKVDEIFGQLRDFYFSVKLSENMKASSFKKLQKFYIDPYKLLPLQRFLPRPPGEKGPPRGGGRGGRGGGRGGGGRGGGRGGGFRGGRGGGGGGFRGGRGGGFRGRGH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | GAR1 ribonucleoprotein homolog (yeast) |
| 别名 | NOLA1, snoRNP protein GAR1 |
| 计算分子量 | 217 aa, 22 kDa |
| 观测分子量 | 28 kDa |
| GenBank蛋白编号 | BC003413 |
| 基因名称 | GAR1 |
| Gene ID (NCBI) | 54433 |
| RRID | AB_2081845 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NY12 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GAR1, other named NOLA1, is a subunit of H/ACA and telomerase. Thought is not required for H/ACA protein assembly, GAR1 is necessary for ribosome biogenesis and telomere. H/ACA involves in class specify the sites of uridine-to-pseudouridine. It contains a core domain that flanked by glycine- and arginine-rich(GAR) domains. GAR1 also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transciptase(TERT) holoenzyme.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for GAR1 antibody 11711-1-AP | Download protocol |
| IP protocol for GAR1 antibody 11711-1-AP | Download protocol |
| WB protocol for GAR1 antibody 11711-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell The human base excision repair enzyme SMUG1 directly interacts with DKC1 and contributes to RNA quality control. | ||
Genome Biol Human dyskerin binds to cytoplasmic H/ACA-box-containing transcripts affecting nuclear hormone receptor dependence | ||
Front Cell Dev Biol PARP1 Regulates the Biogenesis and Activity of Telomerase Complex Through Modification of H/ACA-Proteins. | ||
Mol Cell Biol SUMOylation- and GAR1-Dependent Regulation of Dyskerin Nuclear and Subnuclear Localization. | ||
Pathology Expressional analysis of NOLA1, NOLA2, NOLA3 and DKC1, the core proteins in H/ACA riboproteins, in gastric and colorectal cancers. | ||
Histochem Cell Biol Differential requirements for H/ACA ribonucleoprotein components in cell proliferation and response to DNA damage. |
