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验证数据展示

  • WB analysis using 87287-1-RR (same clone as 87287-1-PBS)

    WB analysis using 87287-1-RR (same clone as 87287-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87287-1-RR (GAP43 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87287-1-PBS in a different storage buffer formulation.

  • WB analysis using 87287-1-RR (same clone as 87287-1-PBS)

    WB analysis using 87287-1-RR (same clone as 87287-1-PBS)

    Fetal human brain tissue was subjected to SDS PAGE followed by western blot with 87287-1-RR (GAP43 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87287-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 87287-1-RR (same clone as 87287-1-PBS)

    IHC staining of mouse brain using 87287-1-RR (same clone as 87287-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 87287-1-RR (GAP43 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87287-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 87287-1-RR (same clone as 87287-1-PBS)

    IF Staining of mouse brain using 87287-1-RR (same clone as 87287-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using GAP43 antibody (87287-1-RR, Clone: 252373G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87287-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 87287-1-RR (same clone as 87287-1-PBS)

    IF Staining of SH-SY5Y using 87287-1-RR (same clone as 87287-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using GAP43 antibody (87287-1-RR, Clone: 252373G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87287-1-PBS in a different storage buffer formulation.

GAP43 Recombinant monoclonal antibody, PBS Only

GAP43 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 87287-1-PBS

产品说明书

CloneNo. 252373G2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

Axonal membrane protein GAP 43, Axonal membrane protein GAP-43, B 50, growth associated protein 43, Growth-associated protein 43

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87287-1-PBS targets GAP43 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9294

Product name: Recombinant human GAP43 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-238 aa of BC007936

Sequence: MLCCMRRTKQVEKNDDDQKIEQDGIKPEDKAHKAATKIQASFRGHITRKKLKGEKKDDVQAAEAEANKKDEAPVADGVEKKGEGTTTAEAAPATGSKPDEPGKAGETPSEEKKGEGDAATEQAAPQAPASSEEKAGSAETESATKASTDNSPSSKAEDAPAKEEPKQADVPAAVTAAAATTPAAEDAAAKATAQPPTETGESSQAEENIEAVDETKPKESARQDEGKEEEPEADQEHA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 growth associated protein 43
别名 Axonal membrane protein GAP 43, Axonal membrane protein GAP-43, B 50, growth associated protein 43, Growth-associated protein 43
计算分子量 238 aa, 25 kDa
观测分子量 43-45 kDa
GenBank蛋白编号BC007936
基因名称 GAP43
Gene ID (NCBI) 2596
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP17677
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The neuronal growth-associated protein GAP43 is known as neuromodulin, B-50, P-57, F1, and pp46. Deficiency of GAP43 in mice results in death early in the postnatal period. GAP43 is one of the main substrates for protein kinase C in the brain. GAP43 is an intracellular growth-associated protein that appears to assist neuronal pathfinding and branching during development and regeneration and may contribute to presynaptic membrane changes in the adult, leading to neurotransmitter release, endocytosis, and synaptic vesicle recycling, long-term potentiation, spatial memory formation, and learning. The predicted molecular weight of about 25 kDa is much lower than the apparent observed molecular weight of 43 kDa on SDS-PAGE gels, and this occurs because the highly charged nature of GAP43 causes it to bind less than the average amount of SDS per amino acid, and because the protein has an elongated structure.

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