验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, NIH/3T3 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:10-1:100 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11054-1-AP targets GANP in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, monkey |
| 免疫原 | GANP fusion protein Ag1486 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | minichromosome maintenance complex component 3 associated protein |
| 别名 | MCM3AP, MAP80, KIAA0572, Germinal-center associated nuclear protein, EC:2.3.1.48 |
| 计算分子量 | 218 kDa |
| 观测分子量 | 75-80 kDa |
| GenBank蛋白编号 | BC013285 |
| 基因名称 | GANP |
| Gene ID (NCBI) | 8888 |
| RRID | AB_2266280 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O60318 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The minichromosome maintenance protein 3 (MCM3) is one of the MCM proteins essential for the initiation of DNA replication. MCM3AP is MCM3 associated protein, and it has phosphorylation-dependent DNA-primase activity, which was up-regulated in antigen immunization induced germinal center. The mutagenesis of a nuclear localization signal of MCM3 affects the binding of this protein with MCM3, suggesting that MCM3AP may also facilitate MCM3 nuclear localization. Besides, MCM3AP is an acetyltransferase cotaining putative acetyl CoA binding motifs conserved within the GCN5-related N-acetyltransferase superfamily.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GANP antibody 11054-1-AP | Download protocol |
| IHC protocol for GANP antibody 11054-1-AP | Download protocol |
| IF protocol for GANP antibody 11054-1-AP | Download protocol |
| IP protocol for GANP antibody 11054-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun GANP regulates recruitment of AID to immunoglobulin variable regions by modulating transcription and nucleosome occupancy.
| ||
J Immunol GANP Interacts with APOBEC3G and Facilitates Its Encapsidation into the Virions to Reduce HIV-1 Infectivity.
| ||
Front Mol Neurosci The Effect of MCM3AP-AS1/miR-211/KLF5/AGGF1 Axis Regulating Glioblastoma Angiogenesis.
| ||
J Biol Chem GANP-mediated recruitment of activation-induced cytidine deaminase to cell nuclei and to immunoglobulin variable region DNA. |
