验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse liver tissue, rat liver tissue, HepG2 cells, SH-SY5Y cells |
| Positive IHC detected in | human liver tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10880-1-AP targets GAMT in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, zebrafish |
| 免疫原 |
CatNo: Ag1327 Product name: Recombinant human GAMT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-236 aa of BC016760 Sequence: MSAPSATPIFAPGENCSPAWGAAPAAYDAADTHLRILGKPVMERWETPYMHALAAAASSKGGRVLEVGFGMAIAASKVQEAPIDEHWIIECNDGVFQRLRDWAPRQTHKVIPLKGLWEDVAPTLPDGHFDGILYDTYPLSEETWHTHQFNFIKNHAFRLLKPGGVLTYCNLTSWGELMKSKYSDITIMFEETQVPALLEAGFRRENIRTEVMALVPPADCRYYAFPQMITPLVTKG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | guanidinoacetate N-methyltransferase |
| 别名 | EC:2.1.1.2, guanidinoacetate N methyltransferase, Guanidinoacetate N-methyltransferase, PIG2, TP53I2 |
| 计算分子量 | 26 kDa |
| 观测分子量 | ~30 kDa |
| GenBank蛋白编号 | BC016760 |
| 基因名称 | GAMT |
| Gene ID (NCBI) | 2593 |
| RRID | AB_2109304 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14353 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GAMT converts the latter to creatine with S-adenosylmethionine as the methyl donor. GAMT was induced in human and mouse cells in response to genotoxic and metabolic stress. GAMT was required for p53-dependent upregulation of creatine biosynthesis, fatty acid oxidation, and apoptosis in response to glucose deprivation.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for GAMT antibody 10880-1-AP | Download protocol |
| WB protocol for GAMT antibody 10880-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Myeloid cell-derived creatine in the hypoxic niche promotes glioblastoma growth | ||
Redox Biol Unbalanced redox status network as an early pathological event in congenital cataracts | ||
Acta Pharm Sin B Spatial metabolomics highlights metabolic reprogramming in acute myeloid leukemia mice through creatine pathway | ||
J Agric Food Chem Artepillin C, a key component of Brazilian propolis, induces thermogenesis in inguinal white adipose tissue of mice through a creatine metabolism-related thermogenic pathway. | ||
Biol Direct GAMT facilitates tumor progression via inhibiting p53 in clear cell renal cell carcinoma | ||
Arthritis Res Ther Investigation of ferroptosis-associated molecular subtypes and immunological characteristics in lupus nephritis based on artificial neural network learning |
