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验证数据展示

  • WB analysis of human liver using 17035-1-AP

    WB analysis of human liver using 17035-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 17035-1-AP (GALT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 17035-1-AP

    IHC staining of human liver using 17035-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17035-1-AP (GALT antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human liver using 17035-1-AP

    IHC staining of human liver using 17035-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17035-1-AP (GALT antibody) at dilution of 1:100 (under 40x lens).

GALT Polyclonal antibody

GALT Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17035-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA and More (1)

别名

Gal 1 P uridylyltransferase, GALT

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17035-1-AP targets GALT in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human
免疫原 GALT fusion protein Ag10524 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 galactose-1-phosphate uridylyltransferase
别名 Gal 1 P uridylyltransferase, GALT
计算分子量 379 aa, 43 kDa
观测分子量 45-48 kDa
GenBank蛋白编号BC015045
基因名称 GALT
Gene ID (NCBI) 2592
RRIDAB_2109296
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP07902
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GALT (galactose-1-phosphate uridylyltransferase) catalyses the second step in the Leloir pathway for the conversion of galactose-l-phosphate (Gal-I-P) into uridine diphosphogalactose (UDP-gal). Defects in GALT cause galactosemia.

实验方案

Product Specific Protocols
WB protocol for GALT antibody 17035-1-APDownload protocol
IHC protocol for GALT antibody 17035-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Inherit Metab Dis

Galactose 1-phosphate accumulates to high levels in galactose-treated cells due to low GALT activity and absence of product inhibition of GALK.

Authors - Sher Li Oh
View Article
humanWB

PLoS One

Angelica Sinensis Polysaccharides Stimulated UDP-Sugar Synthase Genes through Promoting Gene Expression of IGF-1 and IGF1R in Chondrocytes: Promoting Anti-Osteoarthritic Activity.

Authors - Yinxian Wen
View Article
humanWB,IF

Front Mol Biosci

Cytosolic UDP-Gal biosynthetic machinery is required for dimerization of SLC35A2 in the Golgi membrane and its interaction with B4GalT1

Authors - Wojciech Wiertelak
View Article