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验证数据展示

  • WB analysis of human liver using 17035-1-AP

    WB analysis of human liver using 17035-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 17035-1-AP (GALT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 17035-1-AP

    IHC staining of human liver using 17035-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17035-1-AP (GALT antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human liver using 17035-1-AP

    IHC staining of human liver using 17035-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17035-1-AP (GALT antibody) at dilution of 1:100 (under 40x lens).

GALT Polyclonal antibody

GALT Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17035-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Gal 1 P uridylyltransferase, GALT

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

17035-1-AP targets GALT in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 GALT fusion protein Ag10524 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 galactose-1-phosphate uridylyltransferase
别名 Gal 1 P uridylyltransferase, GALT
计算分子量 379 aa, 43 kDa
观测分子量 45-48 kDa
GenBank蛋白编号BC015045
基因名称 GALT
Gene ID (NCBI) 2592
RRIDAB_2109296
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP07902
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GALT (galactose-1-phosphate uridylyltransferase) catalyses the second step in the Leloir pathway for the conversion of galactose-l-phosphate (Gal-I-P) into uridine diphosphogalactose (UDP-gal). Defects in GALT cause galactosemia.

实验方案

Product Specific Protocols
WB protocol for GALT antibody 17035-1-APDownload protocol
IHC protocol for GALT antibody 17035-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Inherit Metab Dis

Galactose 1-phosphate accumulates to high levels in galactose-treated cells due to low GALT activity and absence of product inhibition of GALK.

Authors - Sher Li Oh
View Article
humanWB

PLoS One

Angelica Sinensis Polysaccharides Stimulated UDP-Sugar Synthase Genes through Promoting Gene Expression of IGF-1 and IGF1R in Chondrocytes: Promoting Anti-Osteoarthritic Activity.

Authors - Yinxian Wen
View Article