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GALR2 Polyclonal antibody

GALR2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29909-1-AP

Print datasheet

Synonyms

GAL2 R, galanin receptor 2, Galanin receptor type 2, GALNR2, GALR 2, GALR2

验证数据展示

  • WB analysis using 29909-1-AP

    WB analysis using 29909-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29909-1-AP (GALR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 29909-1-AP

    IHC staining of human pancreas cancer using 29909-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29909-1-AP (GALR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GALR2 antibody included
  • IHC staining of human pancreas cancer using 29909-1-AP

    IHC staining of human pancreas cancer using 29909-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29909-1-AP (GALR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GALR2 antibody included

经过测试的应用

Positive WB detected inHepG2 cells, SMMC-7721 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GALR2 Ready-To-Use IHC Kit. GALR2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29909-1-AP targets GALR2 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human
Immunogen GALR2 fusion protein Ag31762 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name galanin receptor 2
Synonyms GAL2 R, galanin receptor 2, Galanin receptor type 2, GALNR2, GALR 2, GALR2
Calculated Molecular Weight42KD
Observed Molecular Weight 42-50 kDa
GenBank Accession NumberNM_003857
Gene Symbol GALR2
Gene ID (NCBI) 8811
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43603
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Galanin receptor type 2 (GALR2) is a receptor for galanin, GALP, and the hormone SPX1 (PMID:24517231). It is an important neuromodulator present in the brain, gastrointestinal system, and hypothalamo-pituitary axis. Activation of the GALR2 may protect the hippocampus from neuronal damage (PMID: 17263796). It is a 30-amino acid non-C-terminally amidated peptide that potently stimulates growth hormone secretion, inhibits cardiac vagal slowing of heart rate, abolishes sinus arrhythmia, and inhibits postprandial gastrointestinal motility. The activity of this receptor is mediated by G proteins that activate the phospholipase C/protein kinase C pathway (via G(q)) and that inhibits adenylyl cyclase. GALR2 has a calculated molecular weight of 42 kDa.

实验方案

Product Specific Protocols
WB protocol for GALR2 antibody 29909-1-APDownload protocol
IHC protocol for GALR2 antibody 29909-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols