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验证数据展示

  • WB analysis of HEK-293 using 16900-1-AP

    WB analysis of HEK-293 using 16900-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16900-1-AP (GALNTL2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse small intestine using 16900-1-AP

    IHC staining of mouse small intestine using 16900-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16900-1-AP (GALNTL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse small intestine using 16900-1-AP

    IHC staining of mouse small intestine using 16900-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16900-1-AP (GALNTL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 16900-1-AP

    IHC staining of human placenta using 16900-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16900-1-AP (GALNTL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 16900-1-AP

    IHC staining of human placenta using 16900-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16900-1-AP (GALNTL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GALNTL2 Polyclonal antibody

GALNTL2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16900-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

EC:2.4.1.41, GalNAc-T-like protein 2, GALNT15, GALNT7, PIH5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, human liver tissue
Positive IHC detected inmouse small intestine tissue, human kidney tissue, human testis tissue, human placenta tissue, human brain tissue, human lung tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16900-1-AP targets GALNTL2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag10434

Product name: Recombinant human GALNTL2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 287-639 aa of BC014789

Sequence: ECHPGWLEPLLSRIAGDRSRVVSPVIDVIDWKTFQYYPSKDLQRGVLDWKLDFHWEPLPEHVRKALQSPISPIRSPVVPGEVVAMDRHYFQNTGAYDSLMSLRGGENLELSFKAWLCGGSVEILPCSRVGHIYQNQDSHSPLDQETTLRNRVRIAETWLGSFKETFYKHSPEAFSLSKAEKPDCMERLQLQRRLGCRTFHWFLANVYPELYPSEPRPSFSGKLHNTGLGLCADCQAEGDILGCPMVLAPCSDSRQQQYLQHTSRKEIHFGSPQHLCFAVRQEQVILQNCTEEGLAIHQQHWDFQENGMIVHILSGKCMEAVVQENNKDLYLRPCDGKARQQWRFDQINAVDER

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 2
别名 EC:2.4.1.41, GalNAc-T-like protein 2, GALNT15, GALNT7, PIH5
计算分子量 639 aa, 73 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC014789
基因名称 GALNTL2
Gene ID (NCBI) 117248
RRIDAB_2263208
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N3T1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GALNTL2, also known as GALNT15, is a member of the polypeptide ppGalNAc-T family. It catalyzes the initial step in O-linked oligosaccharide biosynthesis by transferring an N-acetyl-D-galactosamine residue to serine or threonine residues on protein receptors. Though it shows weaker activity compared to GALNT2, it can transfer up to seven GalNAc residues to the Muc5AC peptide, suggesting cooperative action with other GALNT proteins. GALNTL2 is widely expressed, with high expression in tissues like the small intestine, placenta, spleen, cerebral cortex, and ovary.

实验方案

Product Specific Protocols
WB protocol for GALNTL2 antibody 16900-1-APDownload protocol
IHC protocol for GALNTL2 antibody 16900-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols