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验证数据展示

  • WB analysis using 24025-1-AP

    WB analysis using 24025-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24025-1-AP (GALNT8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 24025-1-AP

    WB analysis using 24025-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24025-1-AP (GALNT8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

GALNT8 Polyclonal antibody

GALNT8 Polyclonal Antibody for WB, ELISA
Cat No. 24025-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

pp GaNTase 8, GALNAC T8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHCT 116 cells, MCF-7 cells, SH-SY5Y cells, Y79 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24025-1-AP targets GALNT8 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 GALNT8 fusion protein Ag21185 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 8 (GalNAc-T8)
别名 pp GaNTase 8, GALNAC T8
计算分子量 637 aa, 73 kDa
观测分子量73 kDa, 95 kDa
GenBank蛋白编号BC140889
基因名称 GALNT8
Gene ID (NCBI) 26290
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NY28
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GALNT8 (Probable polypeptide N-acetylgalactosaminyltransferase 8) is a member of the UDP-GalNAc polypeptide N-acetylgalactosaminyl transferase (ppGaNTase) family, which initiates mucin-like O-linked protein glycosylation in the Golgi apparatus. The tissue distribution of GALNT8 is high in the heart, skeletal muscle, kidney and liver; moderate in the placenta, small intestine, leukocyte and lung; and weak in the brain, colon, thymus and spleen (PMID: 34576978). The overexpression of GALNT8 significantly promotes the cell cycle and proliferation of CRC cell lines and correlates with poor prognosis in patients with CRC. GALNT8 encodes a 637-amino-acid type-II membrane protein (GalNAc-T8). The detected molecular weight of GALNT8 (95 kDa) is much higher than the theoretical molecular weight (70 kDa) due to three N-GlcNAc sites (N85, N107 and N160) (PMID: 34743989, 34239555).

实验方案

Product Specific Protocols
WB protocol for GALNT8 antibody 24025-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols