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验证数据展示

  • WB analysis using 13671-1-AP

    WB analysis using 13671-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13671-1-AP (GALNT6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 13671-1-AP

    IF Staining of A431 using 13671-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GALNT6 antibody (13671-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

  • IF Staining of A431 using 13671-1-AP

    IF Staining of A431 using 13671-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GALNT6 antibody (13671-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Beta Actin antibody (66009-1-Ig, Clone: 2D4H5, red).

GALNT6 Polyclonal antibody

GALNT6 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 13671-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

EC:2.4.1.41, GALNAC T6, GalNAcT6, GalNAc-T6, Polypeptide N-acetylgalactosaminyltransferase 6

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, MCF-7 cells, T-47D cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13671-1-AP targets GALNT6 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag4652

Product name: Recombinant human GALNT6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 323-622 aa of BC035822

Sequence: FDWSLTFGWETLPPHEKQRRKDETYPIKSPTFAGGLFSISKSYFEHIGTYDNQMEIWGGENVEMSFRVWQCGGQLEIIPCSVVGHVFRTKSPHTFPKGTSVIARNQVRLAEVWMDSYKKIFYRRNLQAAKMAQEKSFGDISERLQLREQLHCHNFSWYLHNVYPEMFVPDLTPTFYGAIKNLGTNQCLDVGENNRGGKPLIMYSCHGLGGNQYFEYTTQRDLRHNIAKQLCLHVSKGALGLGSCHFTGKNSQVPKDEEWELAQDQLIRNSGSGTCLTSQDKKPAMAPCNPSDPHQLWLFV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 6 (GalNAc-T6)
别名 EC:2.4.1.41, GALNAC T6, GalNAcT6, GalNAc-T6, Polypeptide N-acetylgalactosaminyltransferase 6
计算分子量 622 aa, 71 kDa
观测分子量71 kDa
GenBank蛋白编号BC035822
基因名称 GALNT6
Gene ID (NCBI) 11226
RRIDAB_3669168
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NCL4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GALNT6 (polypeptide N-acetylgalactosaminyltransferase 6, GalNAc-T6), which is involved in the initiation of O-glycosylation, has been reported to play crucial roles in mammary carcinogenesis through binding to several substrates (PMID: 32393740). GALNT6 is upregulated in breast, ovarian, renal cell, lung and pancreatic cancers (PMID: 20215525). Overexpression of GRP78 could enhance Golgi-to-ER relocation of GALNT6 (PMID: 28110670).

实验方案

Product Specific Protocols
IF protocol for GALNT6 antibody 13671-1-APDownload protocol
WB protocol for GALNT6 antibody 13671-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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