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验证数据展示

  • IHC staining of mouse pancreas using 17441-1-AP (same clone as 17441-1-PBS)

    IHC staining of mouse pancreas using 17441-1-AP (same clone as 17441-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17441-1-AP (GALNT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17441-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GALNT2 antibody included
  • WB analysis using 17441-1-AP (same clone as 17441-1-PBS)

    WB analysis using 17441-1-AP (same clone as 17441-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17441-1-AP (GALNT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17441-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 17441-1-AP (same clone as 17441-1-PBS)

    IHC staining of human colon using 17441-1-AP (same clone as 17441-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17441-1-AP (GALNT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17441-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GALNT2 antibody included
  • IF Staining of HeLa using 17441-1-AP (same clone as 17441-1-PBS)

    IF Staining of HeLa using 17441-1-AP (same clone as 17441-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GALNT2 antibody (17441-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 17441-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 17441-1-AP (same clone as 17441-1-PBS)

    IF Staining of HeLa using 17441-1-AP (same clone as 17441-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GALNT2 antibody (17441-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 17441-1-PBS in a different storage buffer formulation.

GALNT2 Polyclonal antibody, PBS Only

GALNT2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 17441-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

pp GaNTase 2, Polypeptide N-acetylgalactosaminyltransferase 2 soluble form, Polypeptide N-acetylgalactosaminyltransferase 2, GalNAc-T2, GalNAc T2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy GALNT2 Ready-To-Use IHC Kit. GALNT2 primary antibody included.

产品信息

17441-1-PBS targets GALNT2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag11523

Product name: Recombinant human GALNT2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 222-571 aa of BC041120

Sequence: FLDSHCECNEHWLEPLLERVAEDRTRVVSPIIDVINMDNFQYVGASADLKGGFDWNLVFKWDYMTPEQRRSRQGNPVAPIKTPMIAGGLFVMDKFYFEELGKYDMMMDVWGGENLEISFRVWQCGGSLEIIPCSRVGHVFRKQHPYTFPGGSGTVFARNTRRAAEVWMDEYKNFYYAAVPSARNVPYGNIQSRLELRKKLSCKPFKWYLENVYPELRVPDHQDIAFGALQQGTNCLDTLGHFADGVVGVYECHNAGGNQEWALTKEKSVKHMDLCLTVVDRAPGSLIKLQGCRENDSRQKWEQIEGNSKLRHVGSNLCLDSRTAKSGGLSVEVCGPALSQQWKFTLNLQQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2 (GalNAc-T2)
别名 pp GaNTase 2, Polypeptide N-acetylgalactosaminyltransferase 2 soluble form, Polypeptide N-acetylgalactosaminyltransferase 2, GalNAc-T2, GalNAc T2
计算分子量 571 aa, 65 kDa
观测分子量64 kDa
GenBank蛋白编号BC041120
基因名称 GALNT2
Gene ID (NCBI) 2590
RRIDAB_3085523
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ10471
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GALNT2, a GalNAc-transferase involved in O-linked glycosylation, modulates the risk of atherogenic dyslipidemia, a specific hallmark of insulin resistance and acts as a positive modulator of insulin signaling in human liver cells by down-regulating ENPP1. GALNT2 gene encodes GalNAc-T2 as a regulator of high-density lipoprotein cholesterol (HDL-C) metabolism (PMID: 31040393, 27508872). Western blot analysis detected GALNT2 at an apparent molecular mass of 64 kDa.

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