验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, mouse testis tissue, mouse kidney tissue, mouse lung tissue, rat testis tissue |
| Positive IHC detected in | human kidney tissue, human heart tissue, human liver tissue, human ovary tissue, human skin tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IHC | See 3 publications below |
产品信息
16939-1-AP targets GALNT14 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag10470 Product name: Recombinant human GALNT14 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 203-552 aa of BC010659 Sequence: EVNRDWLQPLLHRVKEDYTRVVCPVIDIINLDTFTYIESASELRGGFDWSLHFQWEQLSPEQKARRLDPTEPIRTPIIAGGLFVIDKAWFDYLGKYDMDMDIWGGENFEISFRVWMCGGSLEIVPCSRVGHVFRKKHPYVFPDGNANTYIKNTKRTAEVWMDEYKQYYYAARPFALERPFGNVESRLDLRKNLRCQSFKWYLENIYPELSIPKESSIQKGNIRQRQKCLESQRQNNQETPNLKLSPCAKVKGEDAKSQVWAFTYTQQILQEELCLSVITLFPGAPVVLVLCKNGDDRQQWTKTGSHIEHIASHLCLDTDMFGDGTENGKEIVVNPCESSLMSQHWDMVSS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 14 (GalNAc-T14) |
| 别名 | GalNac T10, GalNac T14, GALNT14, GALNT15, pp GaNTase 14 |
| 计算分子量 | 552 aa, 64 kDa |
| 观测分子量 | 64 kDa |
| GenBank蛋白编号 | BC010659 |
| 基因名称 | GALNT14 |
| Gene ID (NCBI) | 79623 |
| RRID | AB_2232200 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96FL9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for GALNT14 antibody 16939-1-AP | Download protocol |
| WB protocol for GALNT14 antibody 16939-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncotarget GalNAc-T14 promotes metastasis through Wnt dependent HOXB9 expression in lung adenocarcinoma.
| ||
Mol Cancer Res BORIS expression in ovarian cancer precursor cells alters the CTCF cistrome and enhances invasiveness through GALNT14. | ||
Front Immunol Vessel state and immune infiltration of the angiogenesis subgroup and construction of a prediction model in osteosarcoma | ||
Front Immunol Molecular characterization of immunogenic cell death indicates prognosis and tumor microenvironment infiltration in osteosarcoma
| ||
Cancer Biomark Rs9679162 genotype predicts prognosis of real-world advanced hepatocellular carcinoma treated by sorafenib | ||
Cell Signal GALNT14-mediated O-glycosylation drives lung adenocarcinoma progression by reducing endogenous reactive oxygen species generation |
