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  • Featured Product
  • KD/KO Validated

GALK1 Polyclonal antibody

GALK1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 15284-1-AP

Print datasheet

Synonyms

Galactokinase, galactokinase 1, Galactose kinase, GALK, GALK1, GK1

验证数据展示

  • WB analysis using 15284-1-AP

    WB analysis using 15284-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15284-1-AP (GALK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 15284-1-AP

    IHC staining of human breast cancer using 15284-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15284-1-AP (GALK1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GALK1 antibody included
  • IF Staining of MCF-7 using 15284-1-AP

    IF Staining of MCF-7 using 15284-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 15284-1-AP (GALK1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inMCF-7 cells, mouse heart tissue, Y79 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy GALK1 Ready-To-Use IHC Kit. GALK1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15284-1-AP targets GALK1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman, pig
Immunogen GALK1 fusion protein Ag7403 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name galactokinase 1
Synonyms Galactokinase, galactokinase 1, Galactose kinase, GALK, GALK1, GK1
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC001166
Gene Symbol GALK1
Gene ID (NCBI) 2584
RRIDAB_2108553
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51570
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GALK1, also named as GALK, belongs to the GHMP kinase family and GalK subfamily. GALK1, located on chromosome 17q24, encodes galactokinase and is involved in galactose metabolism (PMID: 32807972). The molecular weight of GALK1 is 42 kDa.

实验方案

Product Specific Protocols
WB protocol for GALK1 antibody 15284-1-APDownload protocol
IHC protocol for GALK1 antibody 15284-1-APDownload protocol
IF protocol for GALK1 antibody 15284-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Proteome Res

Novel Proteomic Biomarker Panel for Prediction of Aggressive Metastatic Hepatocellular Carcinoma Relapse in Surgically Resectable Patients.

Authors - Gek San Tan
  • KD Validated
View Article
pigWB

Eur J Nutr

Epigenetic and SP1-mediated regulation is involved in the repression of galactokinase 1 gene in the liver of neonatal piglets born to betaine-supplemented sows.

Authors - Demin Cai
View Article