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验证数据展示

  • WB analysis using 67772-1-Ig

    WB analysis using 67772-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67772-1-Ig (GALE antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

GALE Monoclonal antibody

GALE Monoclonal Antibody for WB, ELISA
Cat No. 67772-1-Ig

产品说明书

CloneNo. 3F2C2

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse, Rat, Pig, Rabbit

应用

WB, ELISA

别名

FLJ95174, FLJ97302, Galactowaldenase, GALE, SDR1E1, UDP galactose 4 epimerase, UDP glucose 4 epimerase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inU2OS cells, A375 cells, HEK-293T cells, HeLa cells, K-562 cells, 4T1 cells, rat liver tissue, pig liver tissue, rabbit liver tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67772-1-Ig targets GALE in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, Mouse, Rat, Pig, Rabbit
免疫原

CatNo: Ag6571

Product name: Recombinant human GALE protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-348 aa of BC050685

Sequence: MAEKVLVTGGAGYIGSHTVLELLEAGYLPVVIDNFHNAFRGGGSLPESLRRVQELTGRSVEFEEMDILDQGALQRLFKKYSFMAVIHFAGLKAVGESVQKPLDYYRVNLTGTIQLLEIMKAHGVKNLVFSSSATVYGNPQYLPLDEAHPTGGCTNPYGKSKFFIEEMIRDLCQADKTWNAVLLRYFNPTGAHASGCIGEDPQGIPNNLMPYVSQVAIGRREALNVFGNDYDTEDGTGVRDYIHVVDLAKGHIAALRKLKEQCGCRIYNLGTGTGYSVLQMVQAMEKASGKKIPYKVVARREGDVAACYANPSLAQEELGWTAALGLDRMCEDLWRWQKQNPSGFGTQA

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 UDP-galactose-4-epimerase
别名 FLJ95174, FLJ97302, Galactowaldenase, GALE, SDR1E1, UDP galactose 4 epimerase, UDP glucose 4 epimerase
计算分子量 38 kDa
观测分子量 38 kDa
GenBank蛋白编号BC050685
基因名称 GALE
Gene ID (NCBI) 2582
RRIDAB_2918537
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ14376
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GALE(Galactowaldenase) is also named as UDP-glucose 4-epimerase, UDP-galactose 4-epimerase, and belongs to the sugar epimerase family. UDP-galactose 4-epimerase catalyzes the interconversion of UDP-glucose and UDP-galactose during normal galactose metabolism. In humans, deficiencies in this enzyme lead to the complex disorder referred to as epimerase-deficiency galactosemia(PMID:10801319).It can exsit as a homodimer(PMID:8931134).

实验方案

Product Specific Protocols
WB protocol for GALE antibody 67772-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols