验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, NIH/3T3 cells, mouse heart tissue, HEK-293 cells, SKOV-3 cells |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human ovary tumor tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy GADD45GIP1 Ready-To-Use IHC Kit. GADD45GIP1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16260-1-AP targets GADD45GIP1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag9299 Product name: Recombinant human GADD45GIP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-222 aa of BC013027 Sequence: AASVRQARSLLGVAATLAPGSRGYRARPPPRRRPGPRWPDPEDLLTPRWQLGPRYAAKQFARYGAASGVVPGSLWPSPEQLRELEAEEREWYPSLATMQESLRVKQLAEEQKRREREQHIAECMAKMPQMIVNWQQQQRENWEKAQADKERRARLQAEAQELLGYQVDPRSARFQELLQDLEKKERKRLKEEKQKRKKEARAAALAAAVAQDPAASGAPSS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | growth arrest and DNA-damage-inducible, gamma interacting protein 1 |
| 别名 | Growth arrest and DNA damage-inducible proteins-interacting protein 1, CRIF1, CR6-interacting factor 1, CKII beta-associating protein, CKBBP2 |
| 计算分子量 | 222 aa, 25 kDa |
| 观测分子量 | 25-28 kDa |
| GenBank蛋白编号 | BC013027 |
| 基因名称 | GADD45GIP1 |
| Gene ID (NCBI) | 90480 |
| RRID | AB_2108229 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TAE8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for GADD45GIP1 antibody 16260-1-AP | Download protocol |
| IP protocol for GADD45GIP1 antibody 16260-1-AP | Download protocol |
| WB protocol for GADD45GIP1 antibody 16260-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science T cell metabolism. The protein LEM promotes CD8⁺ T cell immunity through effects on mitochondrial respiration. | ||
Cell Mol Life Sci GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation | ||
Oncotarget CR6-interacting factor 1 inhibits invasiveness by suppressing TGF-β-mediated epithelial-mesenchymal transition in hepatocellular carcinoma. | ||
J Mol Med (Berl) Effect of DJ-1 on the neuroprotection of astrocytes subjected to cerebral ischemia/reperfusion injury. | ||
Burns Trauma Cardiac proteomics reveals the potential mechanism of microtubule associated protein 4 phosphorylation-induced mitochondrial dysfunction. | ||
Int J Mol Sci Nmnat1 Deficiency Causes Mitoribosome Excess in Diabetic Nephropathy Mediated by Transcriptional Repressor HIC1 |
