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GADD34 Recombinant antibody

GADD34 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

4K3

Cat No : 81250-1-RR

Print datasheet

Synonyms

GADD34, PPP1R15A

验证数据展示

  • WB analysis of HeLa using 81250-1-RR

    WB analysis of HeLa using 81250-1-RR

    MG132 treated HeLa cells were subjected to SDS PAGE followed by western blot with 81250-1-RR (GADD34 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 81250-1-RR

    WB analysis of HepG2 using 81250-1-RR

    MG132 treated HepG2 cells were subjected to SDS PAGE followed by western blot with 81250-1-RR (GADD34 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inMG132 treated HepG2 cells, MG132 treated NIH/3T3 cells, MG132 treated HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81250-1-RR targets GADD34 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, ELISA
Tested Reactivity Human, Mouse
Immunogen GADD34 fusion protein Ag0578 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 15A
Synonyms GADD34, PPP1R15A
Calculated Molecular Weight 73 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberBC003067
Gene Symbol PPP1R15A
Gene ID (NCBI) 23645
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75807
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GADD34, also named PPP1R15A, belongs to the PPP1R15 family. GADD34 can be triggered as a direct target of activating transcription factor4 (ATF4) under ER stress, it plays a pivotal role in the recovery of cells from shut-down of translation induced by ER stress. It recruits the serine/threonine-protein phosphatase (PP1) to dephosphorylate the translation initiation factor eIF2alpha, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. GADD34 down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 via PP1. It may also promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'. Starvation-induced expression of GADD34 reduced mTOR activity and induced autophagy in wild-type mice, but not in GADD34 KO mice. Molecular weight of GADD34 is 100 kDa confirmed in GADD34 KO mice, and Proteintech's GADD34 antibody 81250-1-RR primarily recongizes the 100 kDa band.

实验方案

Product Specific Protocols
WB protocol for GADD34 antibody 81250-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols