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验证数据展示

  • WB analysis using 10408-1-AP (same clone as 10408-1-PBS)

    WB analysis using 10408-1-AP (same clone as 10408-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10408-1-AP (GAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 10408-1-AP (same clone as 10408-1-PBS)

    IP experiment of mouse brain using 10408-1-AP (same clone as 10408-1-PBS)

    IP result of anti-GAD1 (IP:10408-1-AP, 4ug; Detection:10408-1-AP 1:500) with mouse brain tissue lysate 1920 ug. This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 10408-1-AP (same clone as 10408-1-PBS)

    IHC staining of mouse brain using 10408-1-AP (same clone as 10408-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GAD1 antibody included
  • IHC staining of mouse brain using 10408-1-AP (same clone as 10408-1-PBS)

    IHC staining of mouse brain using 10408-1-AP (same clone as 10408-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GAD1 antibody included
  • IF Staining of mouse brain using 10408-1-AP (same clone as 10408-1-PBS)

    IF Staining of mouse brain using 10408-1-AP (same clone as 10408-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using GAD1 antibody (10408-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10408-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

GAD1 Polyclonal antibody, PBS Only

GAD1 Polyclonal Antibody for WB, IHC, IF-Fro, IP, ELISA
Cat No. 10408-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-Fro, IP, ELISA

别名

GAD67, GAD, GAD 1, GAD 67, GAD-67

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy GAD1 Ready-To-Use IHC Kit. GAD1 primary antibody included.

产品信息

10408-1-PBS targets GAD1 in WB, IHC, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-Fro, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0666

Product name: Recombinant human GAD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-224 aa of BC002815

Sequence: MASSTPSSSATSSNAGADPNTTNLRPTTYDTWCGVAHGCTRKLGLKICGFLQRTNSLEEKSRLVSAFKERQSSKNLLSCENSDRDARFRRTETDFSNLFARDLLPAKNGEEQTVQFLLEVVDILLNYVRKTFDRSTKVLDFHHPHQLLEGMEGFNLELSDHPESLEQILVDCRDTLKYGVRTGHPRFFNQLSTGLDIIGLAGEWLTSTANTNMPSDMRECWLLR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glutamate decarboxylase 1 (brain, 67kDa)
别名 GAD67, GAD, GAD 1, GAD 67, GAD-67
计算分子量 67 kDa
观测分子量 67 kDa
GenBank蛋白编号BC002815
基因名称 GAD1
Gene ID (NCBI) 2571
RRIDAB_2107733
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99259
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The GAD1(glutamate decarboxylase 1) gene encodes 67 kDa glutamic acid decarboxylase isoform (GAD67), the rate-limiting enzyme responsible for γ-aminobutyric acid (GABA) biosynthesis from glutamatic acid and the major GAD isoform in the human brain for early brain development (PMID:21302352). It is also potentially involved in variety of skin activities. It belongs to the group II decarboxylase family.

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